| Cat.No.: | EL-0169 |
| Product Overview: | Human CTSL1 / Cathepsin-L1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer. |
| Species: | Human |
| Source: | Human Cells |
| Lysis Buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Recommended Usage: | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage: | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Gene ID NCBI: | 1514 |
| Official Symbol: | CTSL1 |
| Official Full Name: | cathepsin L1 |
| Synonyms: | CTSL1; cathepsin L1; cathepsin L , CTSL; FLJ31037; cathepsin L; major excreted protein; MEP; CATL; CTSL; |
| mRNA Refseq: | NM_001257971 |
| Protein Refseq: | NP_001244900 |
| MIM: | 116880 |
| UniProt ID: | P07711 |
| Chromosome Location: | 9q21.33 |
| Function: | cysteine-type endopeptidase activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; histone binding; peptidase activity; protein binding; |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0168 | Human CTDSP1 Knockout Cell Line 26bp deletion | Inquiry |
| CL-0195 | Human CTDSPL Knockout Cell Line 1bp insertion | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0169 | Recombinant Human CTSL1 Cell Lysate | Inquiry |
| EL-0204 | Recombinant Human CTCFL 293 Cell Lysate | Inquiry |
| ◆ Antibodies | ||
| EAb-0849 | CTCF Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| CTBP | CTBP2 | CTCF | CTCFL |
| CTDSP1 | CTDSPL | CtIP | ctnnb1 |
| CTSL1 | |||
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