RNA Immunoprecipitation Sequencing (RIP-seq) Service

Traditional methods for studying RNA-protein interactions often face challenges in specificity, throughput, and the ability to capture native complexes. RNA immunoprecipitation sequencing (RIP-seq) overcomes these limitations by combining targeted immunoprecipitation with high-throughput sequencing to provide a genome-wide view of RNA-protein interactions under physiological conditions. CD BioSciences delivers expert end-to-end RIP-seq services, from optimized experimental design to advanced bioinformatic analysis, empowering researchers to uncover critical regulatory networks in development, disease, and fundamental biology with confidence and clarity.

Overview of RIP-seq

RNA immunoprecipitation sequencing (RIP-seq) is a powerful and widely used technique for mapping genome-wide interactions between RNA molecules and specific RNA-binding proteins (RBPs). By employing antibodies to immunoprecipitate a target protein along with its bound RNAs, followed by high-throughput sequencing of the co-precipitated RNA fragments, RIP-seq enables the identification of RNA targets, including mRNAs, lncRNAs, and other non-coding RNAs, with high specificity and sensitivity. Unlike crosslinking-based methods such as CLIP-seq, RIP-seq preserves native RNA-protein interactions without ultraviolet-induced covalent bonds, making it ideal for studying dynamic or transient regulatory complexes. This approach provides critical insights into post-transcriptional gene regulation, including RNA stability, splicing, localization, and translation, and has become an essential tool for elucidating functional mechanisms in development, disease, and RNA biology.

Fig.1 ORF1p RNA Immunoprecipitation. (Briggs E M, et al., 2021)

Features of RIP-seq

RNA immunoprecipitation sequencing (RIP-seq) is a robust discovery tool specifically optimized for mapping the global RNA interactome of a target protein under native physiological conditions. It is ideally suited for studies aiming to identify RNA binding partners without prior crosslinking, making it a preferred method for investigating dynamic or weak RNA-protein interactions, performing unbiased screening for novel RNA targets, and validating RBP functions in diverse biological contexts.

1. Preserves Native Complexes: Avoids crosslinking to maintain RNA-protein interactions in their natural, non-covalent state.
2. Simplified Workflow: Eliminates complex crosslinking and stringent purification steps for a more accessible protocol.
3. Ideal for Discovery Screening: Enables unbiased, genome-wide identification of RNA targets bound by a specific protein.
4. Captures Dynamic Interactions: Sensitive to transient or weak complexes often missed by crosslinking-based methods.

Our Services

CD BioSciences provides expert end-to-end RNA immunoprecipitation sequencing (RIP-seq) services to accurately map the global RNA interactome of your target protein. Utilizing rigorously validated antibodies and optimized native immunoprecipitation protocols, we deliver comprehensive, high-quality data that captures genuine physiological RNA-protein interactions.

Comprehensive RIP-seq Solution

Our RIP-seq service provides a powerful and versatile platform for whole transcriptome analysis of a variety of RNA modifications, such as m6A, m5C, ac4C, Ψ, m7G, and m1A. By employing highly specific antibodies, we can precisely enrich RNA fragments containing target chemical modifications, enabling the identification and quantification of their genomic locations across various RNA species.

Workflow of RIP-seq Service

At CD BioSciences, our end-to-end RIP-seq service follows a rigorously optimized and quality-controlled pipeline to ensure the delivery of reliable and publication-ready data.

Our Advantages

1. Broad Sample Compatibility: Supporting a diverse range of sample types, including adherent/suspension cells, fresh/frozen tissues, and challenging low-input samples through optimized protocols.
2. High Specificity with Rigorously Validated Protocols: Optimized native IP conditions, combined with stringent negative (IgG) and positive controls, ensure high signal-to-noise ratios and reliable identification of true RNA-protein interactions.
3. Proven Technology with Quality Guarantee: Built on established, robust methodologies with stringent QC at every step, guaranteeing high reproducibility, accuracy, and trustworthy data.
4. End-to-End Expert Partnership: Benefit from dedicated guidance by RNA biology specialists, from experimental design through data interpretation, ensuring your scientific questions are effectively addressed.

Understanding the dynamic interactions between RNA and proteins is fundamental to decoding post-transcriptional regulation. With CD BioSciences' comprehensive RIP-seq service, you gain access to precise, reliable, and interpretable data, from rigorous experimental execution to in-depth bioinformatic analysis. Our expert team is ready to partner with you to transform your research vision into impactful discoveries. Contact us today to discuss your project and request a detailed quote.

Reference

1. Briggs E M, McKerrow W, Mita P, et al. RIP-seq reveals LINE-1 ORF1p association with p-body enriched mRNAs[J]. Mobile DNA, 2021, 12(1): 5.