Histone PTM Profiling Service

A comprehensive and unbiased map of histone post-translational modifications (PTMs) is the cornerstone of modern epigenetic discovery, revealing the molecular logic behind gene regulation, cellular identity, and disease. CD BioSciences addresses this foundational need with our advanced histone PTM profiling service. Leveraging high-resolution mass spectrometry and integrated bioinformatics, we provide researchers with a powerful, antibody-independent platform to quantitatively decode the complete histone modification landscape.

Significance of Histone PTM Profiling

Histone post-translational modifications (PTMs) constitute a complex and dynamic molecular language that dictates chromatin structure and function, with direct implications for gene regulation, development, and disease. Profiling these modifications at a systems level is therefore critical; however, traditional antibody-based methods are inherently limited in scope and bias. Histone PTM profiling using high-resolution mass spectrometry provides an unbiased, quantitative, and comprehensive approach to decode this epigenetic landscape. This service enables the discovery of novel modifications, the precise mapping of modification crosstalk, and the systematic identification of disease-associated PTM signatures, forming an essential foundation for hypothesis generation and mechanistic follow-up in modern chromatin biology.

Fig.1 Histone post-translational modifications (PTMs) in chromosome condensation. (Andonegui-Elguera M A, et al., 2022)

Our Services

As a leading provider of integrated epigenetic research services, CD BioSciences offers a professional histone PTM profiling service designed to translate complex biological samples into actionable epigenetic insights. We leverage advanced, high-resolution mass spectrometry platforms coupled with expert bioinformatic analysis to deliver comprehensive, quantitative maps of histone modifications. Our service transforms raw spectral data into clear biological narratives, identifying differential PTM signatures and revealing the epigenetic underpinnings of your experimental conditions, disease states, or developmental processes.

Comprehensive Solution for Histone PTM Profiling

Our service is built upon a high-resolution, high-sensitivity LC-MS/MS platform, enabling unbiased and quantitative analysis of a broad spectrum of histone post-translational modifications (PTMs). This powerful technology allows for the precise detection, relative quantification, and site-specific mapping of diverse modifications, including but not limited to methylation, acetylation, phosphorylation, diverse acylations, ubiquitination, and glycosylation, providing a panoramic view of the epigenetic landscape.

*This list represents our core analytical capabilities. We also support customized targeted analysis for specific modification panels and discovery-driven global profiling to identify novel or unexpected PTM changes.

Workflow of Histone PTM Profiling Service

The basic principle for the identification of histone post-translational modification by mass spectrometry is the change of molecular weight before and after modification. Compared with histones that have not been modified, histones suffered with PTMs hold increased molecular weight at a particular sequence. PTMs of histone can be detected by high-precision mass spectrometry that can distinguish the changes of molecular weight, to further confirm modification type and site. Therefore, as long as we know the precise changes of molecular weight before and after modification, we can help you to identify any histone PTMs.

Fig.2 Workflow of histone PTMs identification.

Samples and Applications

Supported Sample Types

1. Cultured Mammalian Cells: Including adherent or suspension cell lines, as well as primary cells.
2. Tissue Samples: Analysis of fresh, frozen, or FFPE tissues using optimized protocols.
3. Purified Histone Proteins: Direct profiling from acid-extracted or recombinant histone samples.
4. Purified Nucleosomes or Chromatin Complexes: Targeted analysis of histone modifications within assembled chromatin substrates.
5. Yeast and Other Model Organism Cells: Profiling for non-mammalian systems, subject to protocol optimization.
6. Blood-Derived Cells: Such as Peripheral Blood Mononuclear Cells (PBMCs).

Diverse Application Scenarios

1. Drug Mechanism of Action Studies: Analyzing global histone PTM changes in response to epigenetic drug treatments (e.g., HDAC inhibitors, EZH2 inhibitors) to uncover on-target and off-target effects.
2. Disease Biomarker Discovery: Comparing histone PTM landscapes between disease (e.g., cancer, neurological disorders) and healthy control samples to identify potential diagnostic or prognostic epigenetic signatures.
3. Cellular Differentiation & Reprogramming Research: Tracking dynamic reprogramming of the histone modification code during stem cell differentiation, lineage commitment, or cellular reprogramming processes.
4. Environmental & Metabolic Response Profiling: Investigating how environmental stressors, nutrients, or metabolic changes alter the cellular epigenome through specific histone PTM pathways.
5. Basic Chromatin Mechanism Research: Providing unbiased PTM data to support studies on enzyme function (writers/erasers), reader protein specificity, and the crosstalk between different modification types.

Why Choose Us?

1. Unbiased Discovery Power: Enables detection of novel and non-canonical modifications without reliance on specific antibodies.
2. High Resolution & Accuracy: Precisely distinguishes between modification types (e.g., me2 vs. me3) and assigns them to exact amino acid sites.
3. High Quantitative Reproducibility: Ensures reliable differential analysis across samples with excellent technical repeatability.
4. End-to-End Solution: Provides a complete workflow from sample processing to bioinformatic interpretation, lowering the technical barrier for researchers.
5. Seamless Functional Follow-up: Analysis results can directly inform subsequent custom PTM service projects to prepare specific, modified nucleosomes for functional validation.

Deciphering the histone code requires robust, unbiased, and quantitative tools. At CD BioSciences, our histone PTM profiling service delivers precisely that, transforming your complex biological samples into clear, actionable epigenetic insights through high-resolution mass spectrometry and expert analysis. This service not only maps the existing landscape but also opens doors to novel discoveries, providing a critical data foundation for the next generation of chromatin research. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

1. Andonegui-Elguera M A, Cáceres-Gutiérrez R E, López-Saavedra A, et al. The roles of histone post-translational modifications in the formation and function of a mitotic chromosome[J]. International Journal of Molecular Sciences, 2022, 23(15): 8704.