Precisely labeled histones and nucleosomes are fundamental to dissecting the mechanistic details of chromatin biology. At CD BioSciences, we provide expert, end-to-end preparation of these critical tools, integrating a comprehensive suite of advanced technologies to deliver custom, functionally validated substrates for your most demanding research.
At the heart of chromatin and epigenetic regulation lies the nucleosome, the fundamental repeating unit composed of DNA wrapped around a histone protein core. It serves as the central hub where dynamic modifications and interactions dictate gene expression and cellular identity. To mechanistically dissect these complex processes, researchers increasingly rely on functionally labeled histones and nucleosomes—tools that allow precise tracking and interrogation at the molecular level. Site-specific labeling with probes such as fluorophores or biotin enables advanced experimental approaches, including FRET-based conformational studies, single-molecule imaging, interaction pull-down assays, and enzymatic activity profiling. The preparation of these well-defined, homogeneously modified, and functionally intact nucleosomes is therefore a critical prerequisite for cutting-edge research aiming to move beyond correlation and establish direct causation in epigenetic pathways.
Fig.1 Three well-characterized epigenetic mechanisms contribute to non-random mutation and recombination. (Alvarado S G, et al., 2025)
At CD BioSciences, we provide expert, end-to-end preparation of functionally labeled histones and nucleosomes, offering researchers precisely tailored, publication-ready tools to illuminate the mechanisms of epigenetic regulation. Our specialized chemi-biological platform enables site-specific incorporation of probes and modifications, delivering high-purity, structurally defined, and functionally validated nucleosomes for the most demanding biochemical, biophysical, and single-molecule studies.
| Service | Description | Applications |
|---|---|---|
| In Vivo Expression & Labeling | Labeling of histones within living cells using genetically encoded tags (e.g., SNAP-tag) or bioorthogonal chemistry for metabolic incorporation. | Real-time tracking of histone dynamics, turnover, and localization in live cells; studying histone deposition and variant exchange in a native cellular context. |
| Chemical Random Labeling | Conjugation of probes (fluorophores, biotin) to reactive amino acid side chains (e.g., Lys, Cys) on purified histone proteins or assembled nucleosomes. | Rapid feasibility studies, fluorescence-based assays where exact probe position is non-critical, and pull-down experiments requiring bulk labeling. |
| Chemi-Biological Semi-Synthesis | Site-specific ligation of synthetic peptide segments (containing precise labels/modifications) to recombinant protein fragments to generate homogeneously modified, full-length histones or nucleosomes. | High-precision mechanistic studies: FRET-based conformational analysis, single-molecule imaging, structural biology (cryo-EM), and enzymatic activity assays with atomic-level control. |
| Bioorthogonal Chemistry & MS Profiling | Metabolic incorporation of chemical tags into nascent histones in cells, followed by click-chemistry enrichment and mass spectrometric analysis to profile modifications and interactions. | Proteomic discovery of dynamic PTM patterns on newly synthesized histones, identification of interactors specific to nascent chromatin, and cellular-level epigenetic profiling. |
| Recombinant Histone & Nucleosome Production | High-yield expression of wild-type or mutant histones in E. coli and subsequent in vitro assembly of nucleosomes using purified components and client-specified DNA sequences. | Production of foundational, high-purity unlabeled histones and nucleosomes for all downstream applications, custom nucleosome reconstitution for basic biophysical and binding studies. |
In addition to the functionally labeled histones and nucleosomes, CD BioSciences' integrated service platform provides comprehensive support for advanced chromatin research. We offer custom histone & nucleosome PTM service to install defined post-translational modifications, subnucleosomal particle preparation to generate hexasomes and tetrasomes for structural and dynamic studies, and asymmetric nucleosome preparation to reconstitute nucleosomes with differing histone compositions or modifications on each half. Together with our core labeling services, these offerings provide a complete, end-to-end toolkit for mechanistic discovery across biochemistry, biophysics, and structural biology. Contact us today to discuss your specific project requirements with our scientific team.
Reference
1. Alvarado S G, Chang A, Tajerian M. Convergent Evolution and the Epigenome[J]. Epigenomes, 2025, 9(4): 45.
USA
Quick Links
Easy access to products and services you need from our library via powerful searching tools
Privacy Policy | Cookie Policy
Copyright © 2026 CD BioSciences. All Rights Reserved.
