Custom Histone & Nucleosome Preparation Service

Precisely engineered histones and nucleosomes are indispensable for dissecting the molecular mechanisms of chromatin biology and epigenetic regulation. CD BioSciences provides a comprehensive and fully integrated custom histone & nucleosome preparation service, designed to transform your specific and complex research requirements into well-defined, functional reality. Our platform consolidates advanced capabilities across chemical synthesis, protein engineering, and DNA assembly, delivering the exact, high-fidelity substrates you need to advance from observation to mechanistic discovery.

Significance of Histone & Nucleosome Preparation

Histones are the core protein components around which DNA is wrapped to form nucleosomes, the fundamental repeating units of chromatin. As the primary substrate for epigenetic regulation, nucleosomes and their modifications directly control DNA accessibility and gene expression. Preparing well-defined, homogenous histones and nucleosomes in vitro is therefore a foundational step for mechanistic research. It allows scientists to move beyond cellular complexity and dissect, with molecular precision, how specific histone variants, post-translational modifications, and structural changes drive epigenetic signaling, chromatin dynamics, and disease states.

Fig.1 Linker histone H1 regulates homeostasis of heterochromatin-associated cRNAs. (Bujosa P, et al., 2024)

Our Services

CD BioSciences provides customized histone and nucleosome preparation service, offering a fully integrated platform for your most demanding chromatin research needs. We go beyond offering discrete options by combining our core expertise in protein engineering, chemical synthesis, and DNA assembly into a unified project-based workflow. This allows us to transform your complex design concepts, including those involving specific PTMs, asymmetric architectures, functional labels, or specialized histone variants, into well-defined, functional reality. We deliver the precise molecular tools required for definitive mechanistic discovery.

One-stop Solution for Histone & Nucleosome Preparation

To empower the next generation of discovery in chromatin biology, CD BioSciences offers a complete, integrated suite of preparation services. Our platform consolidates expertise across the full spectrum of molecular engineering required for epigenetics, from fundamental component assembly to the construction of highly sophisticated and defined chromatin substrates.

Functionally Labeled Histone and Nucleosome Preparation Service

This service enables the site-specific incorporation of functional probes—such as fluorophores for FRET, biotin for pulldowns, or non-natural amino acids—into histones and nucleosomes. It provides the essential, homogeneously labeled substrates required for high-sensitivity detection and real-time monitoring in single-molecule imaging, binding studies, and advanced biochemical assays.

Custom Histone & Nucleosome PTM Service

We specialize in the precise, site-specific installation of single or combinatorial post-translational modifications (PTMs), including methylation, acetylation, phosphorylation, and ubiquitination. This service delivers nucleosomes with a chemically defined PTM landscape, which are critical for unambiguous studies on epigenetic enzyme mechanisms, reader protein specificity, and the biochemical consequences of specific modification patterns.

Subnucleosomal Particle Preparation Service

Focusing on chromatin intermediates, this service provides the preparation of structurally defined hexasomes and tetrasomes. These homogeneous particles, which lack one or both H2A-H2B dimers, serve as ideal model systems for mechanistic studies on histone chaperone function, chromatin remodeling dynamics, and the stepwise assembly/disassembly pathways of the nucleosome.

Asymmetric Nucleosome Preparation Service

This advanced service constructs nucleosomes with programmed differences between their two halves, such as distinct histone variants or PTM patterns on each side. It offers a unique in vitro model to investigate biologically relevant asymmetric states, enabling research into directional processes in epigenetics, including oriented binding of reader proteins and the polarized activity of remodeling complexes.

Workflow of Histone & Nucleosome Preparation Service

Research Applications

1. Mechanistic Studies of Chromatin-Associated Enzymes
2. High-Resolution Structural Biology Research
3. Single-Molecule Biophysics & Imaging Research

1. Interaction Analysis & Proteomic Screening
2. Drug Discovery & Therapeutic Development
3. Fundamental Chromatin Dynamics & Engineering

Why Choose Us?

1. Unified Technical Mastery: A single integrated platform expertly combines chemical synthesis, protein engineering, and DNA assembly, eliminating the need to coordinate multiple vendors for complex projects.
2. End-to-End Project Ownership: Our dedicated scientists provide comprehensive support from initial design to final validation, acting as your accountable partner throughout the entire workflow.
3. Commitment to Precision & Reproducibility: We deliver homogenous, rigorously characterized products with guaranteed batch-to-batch consistency, forming a reliable foundation for high-impact, publishable data.
4. Accelerated Path to Discovery: Our specialized expertise and optimized processes save you months of method development and troubleshooting, allowing you to focus resources on experimentation and scientific insight.

Navigating the complexities of chromatin biology demands tools of the highest precision and reliability. CD BioSciences stands as your dedicated partner in this endeavor, providing an unparalleled, integrated platform for custom histone and nucleosome preparation. From fundamental enzymatic studies to cutting-edge structural and single-molecule research, we transform your most challenging conceptual designs into definitive molecular tools, empowering you to ask and answer the next generation of questions in epigenetics. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

1. Bujosa P, Reina O, Caballé A, et al. Linker histone H1 regulates homeostasis of heterochromatin-associated cRNAs[J]. Cell Reports, 2024, 43(5).