Histone & Nucleosome Analysis Service

Histones and nucleosomes form the fundamental architecture of chromatin, dynamically regulating gene expression and genome integrity through their composition, modifications, and higher-order organization. CD BioSciences offers a comprehensive, integrated suite of analytical services—from high-resolution PTM mapping and genome-wide positioning to real-time dynamics and structural biology—to empower your research in decoding the epigenetic mechanisms underlying development, disease, and cellular function.

Overview of Histone & Nucleosome

Histones are the fundamental protein components of chromatin, responsible for packaging DNA into structural units called nucleosomes—the repeating core particles of chromatin. Each nucleosome consists of approximately 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3, H4). This organization not only compacts the genome but also serves as a dynamic platform for a vast array of post-translational modifications (PTMs) and epigenetic regulation. The precise composition, modification state, and positioning of nucleosomes are critical determinants of DNA accessibility, gene expression, DNA repair, and ultimately, cellular function and identity.

Fig.1 Schematic diagram of the structure of a nucleosome and its location in chromatin. (Dutta S, et al., 2025)

Significance of Histone & Nucleosome Analysis

Analysis of histones and nucleosomes provides fundamental insights into the epigenetic regulation of gene expression and genome function, serving as a critical bridge between DNA sequence information and cellular phenotype. By characterizing histone composition, post-translational modifications (PTMs), nucleosome positioning, and higher-order chromatin architecture, researchers can decode the dynamic regulatory mechanisms that control cellular identity, response to stimuli, and disease pathogenesis.

1. Deciphering the Epigenetic Code: Understanding how specific histone PTMs and their combinations ("histone code") influence gene activity and silencing.
2. Elucidating Chromatin Dynamics: Revealing how nucleosome positioning, remodeling, and histone variant incorporation regulate DNA accessibility for transcription, replication, and repair.
3. Uncovering Disease Mechanisms: Identifying aberrant histone modifications or nucleosome organization as drivers in cancer, neurological disorders, metabolic diseases, and autoimmune conditions.
4. Advancing Therapeutic Development: Discovering novel epigenetic drug targets and developing biomarkers for diagnosis, prognosis, and monitoring treatment response.
5. Integrating Multi-Omics Data: Providing a crucial layer of molecular context to integrate with genomic, transcriptomic, and proteomic datasets for systems-level biology.

Our Services

At CD BioSciences, we provide an integrated and comprehensive suite of analytical services dedicated to the characterization of histones and nucleosomes. Our platform combines advanced mass spectrometry, next-generation sequencing, and biophysical techniques to deliver end-to-end solutions—from the precise mapping of histone post-translational modifications (PTMs) and variants to the high-resolution analysis of nucleosome composition, positioning, and dynamics.

One-stop Solution for Histone & Nucleosome Analysis

Whether you are exploring fundamental chromatin biology, investigating disease mechanisms, or developing epigenetic therapeutics, our tailored services are designed to generate robust, publication-ready data that empower your research.

1. Core Technology Matrix

1. Featured Service Modules

Custom Histone & Nucleosome Preparation Service

We provide tailored services for the expression, purification, and in vitro reconstitution of recombinant histones and nucleosomes with specified modifications or variants, supplying high-purity materials for your biochemical and structural studies.

1. Functionally Labeled Histone and Nucleosome Preparation Service
2. Custom Histone & Nucleosome PTM Service
3. Subnucleosomal Particle Preparation Service
4. Asymmetric Nucleosome Preparation Service

Histone PTM Profiling Service

Utilizing high-resolution mass spectrometry, we offer comprehensive qualitative and quantitative analysis of histone post-translational modifications, enabling precise mapping of the epigenetic landscape under various biological conditions.

1. Lysine/Arginine Methylation Analysis
2. Lysine Acetylation Analysis
3. Lysine Acylation Analysis
4. Lysine Crotonylation Analysis
5. Lysine Lactylation Analysis
6. Lysine Formylation Analysis

1. Lysine Ubiquitination Analysis
2. Lysine SUMOylation Analysis
3. Lysine Succinylation Analysis
4. Serine/Threonine/Tyrosine Phosphorylation Analysis
5. Arginine Citrullination Analysis
6. Serine/Threonine O-GlcNAcylation Analysis

Chromatin Remodeling Assay Service

Our service integrates functional activity characterization with high-resolution structural analysis. We utilize biochemical and single-molecule methods to quantify the dynamic activity of chromatin remodeling factors, including nucleosome sliding, expulsion, and histone exchange. To directly link function and mechanism, the service incorporates cryo-electron microscopy for chromatin remodeling complex analysis, enabling near-atomic resolution observation of remodeling factor-nucleosome interactions and revealing the structural basis of ATP-dependent chromatin remodeling.

smFRET for Nucleosome Dynamics Research

We employ single-molecule fluorescence resonance energy transfer (smFRET) to probe the real-time structural dynamics and energy landscape of nucleosomes. This service quantitatively measures conformational changes, stability, and assembly/disassembly kinetics at the single-particle level, providing mechanistic insights into how histone variants, PTMs, and binding partners regulate chromatin accessibility and function.

Cryo-EM for Nucleosome Complex Structure Analysis

Using cryo-electron microscopy (cryo-EM), we determine high-resolution structures of nucleosomes in complex with readers, writers, erasers, or remodelers. This service captures structural details at near-atomic resolution (typically 3–4 Å), revealing precise interaction interfaces, allosteric changes, and the molecular basis of epigenetic regulation within physiologically relevant complexes.

Workflow of Histone & Nucleosome Analysis Service

Our Advantages

1. Integrated Multi-Technology Platform: We uniquely combine mass spectrometry, sequencing, biophysics, and structural biology for comprehensive histone and nucleosome analysis in a single workflow.
2. Expertise in Complex Sample Preparation: We specialize in handling challenging samples, from low-input clinical specimens to custom-designed asymmetric nucleosomes and chromatin complexes.
3. End-to-End Scientific Partnership: Our specialists provide collaborative support from experimental design through data interpretation, ensuring each project is fully customized to your goals.
4. Rigorous, Publication-Ready Data: We adhere to stringent quality controls to deliver robust, reproducible results, complete with the documentation and validation needed for publication.

At CD BioSciences, we are dedicated to providing cutting-edge, integrated solutions for histone and nucleosome analysis. By combining advanced technologies with deep scientific expertise, we transform complex chromatin biology questions into clear, actionable insights. Our commitment to quality, customization, and collaborative partnership ensures that you receive the precise data and support needed to advance your research in epigenetics, disease mechanisms, and therapeutic discovery. Partner with us to unlock the next level of understanding in chromatin regulation.

Reference

1. Dutta S, Dutta S, Somanath P R, et al. Circulating nucleosomes and histones in the development of lung injury and sepsis[J]. Current Issues in Molecular Biology, 2025, 47(2): 133.