Histone Lysine Ubiquitination Analysis

Histone lysine ubiquitination is a crucial regulatory mechanism influencing chromatin dynamics and gene expression. CD BioSciences offers advanced histone lysine ubiquitination analysis services, providing precise mapping and quantification to help you uncover the functional roles of this complex modification in cellular processes and disease mechanisms. Our tailored solutions deliver reliable, publication-ready data to accelerate your epigenetic research.

Overview of Histone Lysine Ubiquitination

Histone lysine ubiquitination involves the covalent attachment of ubiquitin, a 76-amino acid protein, to specific lysine residues on histone tails. This dynamic and reversible modification can occur as monoubiquitination or polyubiquitination, with different chain topologies (e.g., K48, K63) dictating distinct functional outcomes. Biologically, it plays pivotal roles in transcriptional regulation (notably H2B ubiquitination in activation), DNA damage repair (through recruitment of repair complexes), cell cycle progression, and signal transduction. Dysregulation of histone ubiquitination is increasingly linked to cancer, neurodegenerative disorders, and developmental abnormalities, highlighting its significance as a key epigenetic regulator and potential therapeutic target.

Fig.1 E3 ubiquitin ligases and histone ubiquitination events. (Oss-Ronen L, et al., 2022)

Our Services

CD BioSciences specializes in end-to-end histone lysine ubiquitination analysis, combining high-resolution mass spectrometry with optimized enrichment strategies to address the challenges of low abundance and structural complexity. Our services provide reliable identification, precise quantification, and functional insights, empowering researchers to investigate ubiquitination dynamics across diverse biological contexts and accelerate discoveries in epigenetics and disease biology.

Customized Solution for Histone Lysine Ubiquitination Analysis

At CD BioSciences, we provide tailored analytical solutions targeting key histone lysine ubiquitination events. Our services cover a range of well-characterized modifications to support your specific research focus:

Ubiquitination Modification Types

1. H2B Ubiquitination
2. H2A Ubiquitination
3. H2A.Z Ubiquitination
4. H3 Ubiquitination

1. H4 Ubiquitination
2. Polyubiquitin Chains
3. Multi-Site Ubiquitination
4. Other Ubiquitination Modifications

Comprehensive Analysis Services

1. Global Ubiquitinome Profiling: Comprehensive identification and mapping of ubiquitination sites across all core histones.
2. Site-Specific Quantification: Targeted relative or absolute quantification of specific ubiquitination events (e.g., H2BK120ub, H2AK119ub).
3. Dynamic Monitoring: Time-course or stimulus-response studies to track ubiquitination changes.
4. Integrated Multi-PTM Analysis: Combined profiling of ubiquitination with other modifications (e.g., acetylation, methylation) for systems-level insights.

Workflow of Histone Lysine Ubiquitination Analysis Service

Supported Sample Types

Our service is optimized for a wide range of biological materials. Each sample type is processed using tailored protocols to ensure the integrity of ubiquitination modifications.

1. Mammalian Cell Lines: Including both adherent and suspension cultures.
2. Primary Cells & Blood-Derived Cells: Such as PBMCs, lymphocytes, and other isolated primary cells.
3. Fresh/Frozen Animal Tissues: Tumor biopsies, organ samples, and other tissue specimens.
4. Plant & Fungal Samples: Processed using case-specific protocols developed for challenging cell wall structures and unique histone variants.
5. Pre-Extracted Histones: Purified histone extracts from any biological source.
6. FFPE Tissue Sections: Archival samples are accepted subject to a feasibility assessment to evaluate ubiquitination preservation.

Our Advantages

1. Specialized Enrichment & Capture: We employ optimized, multi-strategy protocols to efficiently isolate ubiquitinated histones, ensuring high recovery even for low-abundance or labile modifications.
2. High-Fidelity Ubiquitin Chain Analysis: Our platform precisely distinguishes mono- from polyubiquitination and characterizes specific chain linkage types (e.g., K48 vs. K63), providing critical functional insights.
3. Gold-Standard Absolute Quantification: Using stable isotope-labeled internal standards, we deliver definitive, publication-ready molar quantification—not just relative changes—of specific ubiquitination events.
4. End-to-End Expert Partnership: Your project is supported by our PhD-level epigenetics specialists from initial consultation and experimental design through to in-depth data interpretation and biological context.

At CD BioSciences, our expertise extends beyond histone ubiquitination to offer a full spectrum of epigenetic analysis services. We provide comprehensive solutions for studying acetylation, methylation, phosphorylation, formylation, succinylation, crotonylation, lactylation, and SUMOylation, among other modifications. Our flexible, custom-tailored approaches ensure you receive integrated, publication-ready data to advance your research in chromatin biology and translational medicine. Contact us to develop a personalized strategy for your next project.

Reference

1. Oss-Ronen L, Sarusi T, Cohen I. Histone mono-ubiquitination in transcriptional regulation and its mark on life: emerging roles in tissue development and disease[J]. Cells, 2022, 11(15): 2404.