Histone Lysine Succinylation Analysis

Succinylation is a key histone modification driven by the metabolite succinyl-CoA, directly linking cellular metabolic states to gene expression regulation. CD BioSciences provides comprehensive histone lysine succinylation analysis services based on high-resolution mass spectrometry, helping to reveal its functional mechanisms in metabolic reprogramming, disease development, and signal transduction.

Overview of Histone Lysine Succinylation

Histone lysine succinylation is a post-translational modification characterized by the covalent addition of a succinyl group (-CO-CH2-CH2-CO-) to specific lysine residues. This reaction is primarily catalyzed by succinyl-CoA, a central metabolite in the tricarboxylic acid (TCA) cycle. The addition of this bulky, negatively charged moiety neutralizes the lysine's positive charge, leading to significant alterations in chromatin structure and electrostatic interactions.

Biologically, succinylation serves as a direct sensor of cellular metabolic state, influencing key processes such as gene transcription, DNA repair, and cellular differentiation. Dysregulated succinylation has been implicated in various pathologies, including cancer (where mutations in succinate dehydrogenase lead to succinate accumulation), cardiovascular diseases, and metabolic syndromes, highlighting its role as a critical interface between metabolism and epigenetics.

Fig.1 The role of lysine succinylation in gene expression. (Zheng Z, et al., 2025)

Our Services

To address the unique challenges of studying this labile and metabolism-sensitive modification, CD BioSciences provides tailored histone lysine succinylation analysis services. We combine advanced mass spectrometry with optimized enrichment strategies to deliver accurate identification and robust quantification, empowering you to explore succinylation's role in metabolic adaptation and disease mechanisms.

One-stop Solution for Histone Lysine Succinylation Analysis

Our integrated platform offers a complete workflow for in-depth investigation of histone lysine succinylation, from unbiased discovery to targeted mechanistic studies. We provide tailored solutions to help you explore the dynamic interplay between metabolic states and chromatin regulation.

Workflow of Histone Lysine Succinylation Analysis Service

At CD BioSciences, our specialized workflow is designed to ensure the accurate capture and analysis of the dynamic histone succinylation modification, directly linking epigenetic data to metabolic context.

Project Consultation & Design

We begin with a collaborative consultation to align the analytical strategy with your metabolic research goals. Together, we define the experimental scope—whether global profiling or targeted quantification—and design the study to effectively capture succinylation dynamics.

Metabolism-Preserving Sample Preparation

Histones are extracted using gentle, non-denaturing protocols to preserve labile succinylation states. Samples then undergo specific enrichment to isolate succinylated peptides, significantly enhancing detection sensitivity for downstream analysis.

High-Resolution LC-MS/MS Analysis

Samples are analyzed via nanoLC coupled to high-resolution tandem mass spectrometry. Our optimized acquisition methods, such as PRM or DIA, are fine-tuned for the specific detection and accurate quantification of succinylated peptides.

Data Processing & Integrated Bioinformatics

Raw data is processed through a dedicated pipeline for precise site localization and statistical quantification. The results can be integrated with metabolic pathway databases to link succinylation changes directly to alterations in cellular metabolism.

Comprehensive Reporting & Interpretation

You receive a detailed report containing all data, annotated spectra, and clear visualizations. Our experts provide contextual biological interpretation, linking the succinylation profile to potential metabolic functions and regulatory mechanisms.

Supported Sample Types

We support a wide range of biological specimens to accommodate diverse research models, with a particular focus on samples relevant to metabolic and translational studies.

Standard Samples

1. Mammalian Cell Lines: including standard cultured adherent or suspension cells, as well as cells subjected to metabolic interventions (e.g., glucose deprivation, hypoxia, or mitochondrial inhibitor treatment).
2. Primary Cells and Tissues: fresh or frozen samples, including liver, heart, and tumor biopsy samples.
3. Pre-extracted Histones: purified histones from any biological source, available for direct analysis.

Special Samples

1. Clinical & Metabolic Phenotype Samples: Tissues or cells from models or cases with aberrant serum/tissue succinate levels or other metabolic disorders.
2. Plant and Microbial Samples: Specimens from photosynthetic organisms, bacterial cultures, and other non-mammalian models studied under customized protocols.
3. Archival FFPE Tissue Sections: Formalin-fixed paraffin-embedded samples, accepted subject to a prior feasibility assessment for modification preservation.

Our Advantages

1. Metabolism-Sensitive Sample Processing: Our optimized, gentle extraction protocols are designed to preserve labile succinylation states by minimizing enzymatic and chemical degradation, ensuring an accurate snapshot of in vivo modification levels.
2. Charge-Based Specificity & Enrichment: We leverage the unique negative charge introduced by succinylation through tailored enrichment or separation strategies. This approach significantly enhances detection specificity and sensitivity by effectively isolating succinylated peptides from complex backgrounds.
3. Gold-Standard Absolute Quantification: We provide definitive, publication-ready quantification using stable isotope-labeled succinylated peptide internal standards. This allows for precise measurement of the molar abundance of specific modifications, moving beyond relative changes.
4. Multi-Dimensional Metabolic Context Analysis: Our service can be integrated with complementary analyses, such as succinyl-CoA level measurement or targeted metabolomics, to directly correlate changes in histone succinylation with shifts in cellular metabolic flux and pathway activity.

Our expertise is not limited to succinylation. CD BioSciences offers comprehensive analysis services for a wide spectrum of histone PTMs, including acetylation, methylation, phosphorylation, ubiquitination, SUMOylation, formylation, crotonylation, lactylation, and more. Whether you need focused investigation of a single modification or a customized, multi-dimensional PTM profiling study, our flexible platform and scientific partnership ensure your research questions are answered with precision and depth. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

1. Zheng Z, Xiao P, Kuang J, et al. Unlocking the Hidden Potential of Cancer Therapy Targeting Lysine Succinylation[J]. Journal of Cancer, 2025, 16(3): 821.