Histone Lysine Lactylation Analysis

Histone lysine lactylation (Kla) serves as a direct molecular link between cellular metabolic flux and epigenetic regulation, offering critical insights into immune reprogramming, tumor metabolism, and neural plasticity. CD BioSciences specializes in deciphering this metabolic-epigenetic crosstalk through advanced histone lysine lactation analysis services, offering precise detection and dynamic quantification to accelerate discovery in immunometabolism, oncology, and metabolic disease research.

Introduction to Histone Lysine Lactylation

Histone lysine lactylation (Kla) is a metabolite‑derived post‑translational modification in which lactate—an end‑product of glycolysis and a key metabolic signaling molecule—is covalently attached to specific lysine residues on histone tails. This modification directly couples cellular metabolic state, such as aerobic glycolysis (the Warburg effect) or hypoxia, to chromatin‑mediated gene regulation. Kla has been shown to promote anti‑inflammatory gene expression in macrophages, drive oncogenic transcriptional programs in cancer cells, and modulate synaptic plasticity in neurons. Studying lactylation therefore provides a unique window into how metabolic changes reprogram cell fate and function in health and disease, with significant implications for immunotherapy, cancer therapeutics, and metabolic disorder research.

Fig.1 Lactate metabolism and histone lysine lactylation in cancer cells. (Xu X, et al., 2023)

Our Services

With a focus on metabolic-epigenetic interplay, CD BioSciences delivers end‑to‑end histone lysine lactation analysis services designed to address the specific challenges of working with this dynamic, metabolism‑sensitive mark. We combine high‑resolution mass spectrometry optimized for lactylation detection with tailored bioinformatics to generate reliable, publication‑quality data.

Customized Solution for Histone Lysine Lactylation Analysis

Our solutions are based on a metabolically optimized analysis platform that integrates high-sensitivity mass spectrometry and lactation-specific bioinformatics. Whether your goal is to map new lactation sites, quantify Kla dynamics under different metabolic conditions, or integrate lactation profiles with functional genomics datasets, our services provide the precision and biological insights needed to advance your research.

1. Global Lactylome Mapping: Comprehensive identification and profiling of lactylation sites across histone proteins to explore novel regulatory landscapes.
2. Targeted Kla Quantification: High-precision, quantitative analysis of specific lactylation marks (e.g., H3K18la) to monitor dynamics across experimental conditions.
3. Spatiotemporal Dynamics Analysis: Tracking lactylation changes over time or across different cellular compartments in response to metabolic cues.
4. Integrated Multi-Omics Analysis: Correlating lactylation profiles with transcriptomic or metabolomic datasets to establish functional gene regulatory networks.
5. Mechanistic Validation Support: Providing downstream analysis and consultation to help link specific lactylation events to phenotypic outcomes.

Workflow of Histone Lysine Lactylation Analysis Service

Our Advantages

1. Metabolism-Specialized Expertise: Our analytical approach is guided by deep knowledge in both epigenetic mechanisms and cellular metabolism, enabling precise experimental design and biologically relevant interpretation of lactylation data.
2. Extensive Sample Compatibility: We support diverse sample types central to lactylation research—including primary immune cells, tumor tissues, neural cultures, and metabolically engineered models—accommodating a wide range of experimental designs.
3. High-Fidelity Analytical Sensitivity: Our optimized platform combines metabolite-stabilizing sample preparation with high-resolution mass spectrometry to reliably detect and quantify lactylation even at low stoichiometry.
4. Functionally Integrated Data Delivery: We provide not just modification maps, but pathway-enriched analyses and mechanistic hypotheses that directly connect lactylation patterns to metabolic states and phenotypic outcomes.

Beyond lactylation analysis, CD BioSciences supports a full spectrum of histone PTM investigations—including methylation, acetylation, phosphorylation, crotonylation, ubiquitination, and more—with the same level of technical rigor and biological insight. Whether you are characterizing a novel modification or building a systems-level understanding of epigenetic regulation, our tailored approaches are designed to meet your specific research objectives. Contact us today to advance your research with precision and confidence.

Reference

1. Xu X, Peng Q, Jiang X, et al. Metabolic reprogramming and epigenetic modifications in cancer: from the impacts and mechanisms to the treatment potential[J]. Experimental & molecular medicine, 2023, 55(7): 1357-1370.