Histone lysine crotonylation (Kcr) has emerged as a vital epigenetic regulator with distinct functions in transcriptional activation, development, and disease. To fully unravel its biological impact, precise and reliable detection is essential. CD BioSciences offers expert histone lysine crotonylation analysis services, delivering accurate mapping, quantification, and functional interpretation to advance your research in epigenetics and beyond.
Histone lysine crotonylation (Kcr) is a recently identified acylation mark characterized by the addition of a crotonyl group to specific lysine residues. Unlike the more common acetylation, crotonylation is strongly associated with active transcription, particularly at promoters and enhancers of highly expressed genes. It plays critical roles in processes such as spermatogenesis, embryonic development, stem cell pluripotency, and cellular response to metabolic changes. Dysregulation of Kcr has been linked to cancers, neurological disorders, and metabolic diseases, making it a promising target for therapeutic exploration and a valuable focus for epigenetic research.
Fig.1 Histone lysine crotonylation is an evolutionarily conserved post-translational epigenetic modification. (Stein R A, Riber L., 2023)
As a leading provider of epigenetic research services, CD BioSciences offers end-to-end histone lysine crotonylation (Kcr) analysis service tailored to the specific needs of epigenetics researchers. We integrate cutting-edge technology platforms with deep scientific expertise to deliver reliable, publication-ready data. Whether your goal is to explore novel Kcr sites, quantify dynamic modification changes, or integrate crotonylation data with other omics layers, we provide customized solutions designed to accelerate scientific discovery and support hypothesis-driven research.
One-stop Solution for Histone Lysine Crotonylation Analysis
Using high-resolution mass spectrometry with our optimized analytical pipeline, we achieve precise discrimination of crotonylation from structurally similar modifications (particularly acetylation), enable sensitive detection of low-abundance Kcr sites, and deliver biologically interpretable insights from complex datasets. By leveraging immunoaffinity enrichment strategies and our curated spectral library, we ensure precise identification, reliable quantification, and deep functional insights into this dynamic epigenetic mark.
| Service | Description | Primary Application |
|---|---|---|
| Global Crotonylome Profiling | Unbiased identification and mapping of histone Kcr sites across the entire proteome. | Discovery of novel crotonylation sites and initial landscape characterization. |
| Targeted Kcr Quantification | High-precision, reproducible absolute or relative quantification of specific, pre-defined Kcr marks (e.g., H3K4cr, H3K9cr). | Validation and differential analysis across experimental conditions (e.g., disease vs. control, drug treatment). |
| Site-Specific Dynamics Analysis | Monitoring temporal or spatial changes in crotonylation levels at individual loci. | Studying signaling pathways, developmental stages, or cellular responses. |
| Multi-Omics Integration | Correlating crotonylation data with transcriptomic (RNA-seq) or other epigenetic datasets. | Uncovering functional links between Kcr marks and gene expression or other regulatory layers. |
We initiate each project with a collaborative consultation to thoroughly understand your biological model and scientific objectives. Based on this, we co-design a customized analysis strategy, selecting the most appropriate service module (e.g., global profiling vs. targeted quantification) and defining key experimental parameters.
Your samples are processed using our optimized, standardized protocols for histone acid extraction, enzymatic digestion, and peptide purification. Each batch includes rigorous internal quality controls to assess histone purity, digestion efficiency, and peptide yield, ensuring optimal input for downstream mass spectrometry.
Peptide samples are analyzed on our state-of-the-art high-resolution mass spectrometry platforms. We employ methods specifically optimized for acylation analysis, including parallel reaction monitoring (PRM) for targeted quantification or data-independent acquisition (DIA) for comprehensive profiling, ensuring precise detection and discrimination of crotonylation.
The raw MS data is processed through our proprietary bioinformatics pipeline. This encompasses accurate crotonylation site identification and localization, stringent false discovery rate control, precise relative/absolute quantification, differential analysis between sample groups, and advanced functional annotation through pathway and motif enrichment analyses.
We deliver a detailed, publication-ready report containing all experimental results, high-quality visualizations (volcano plots, heatmaps), and clear biological interpretation. The package includes all raw and processed data files. Our scientists provide subsequent consultation to discuss findings, assist with result contextualization, and support further experimental planning.
CD BioSciences is your trusted partner not only for histone crotonylation analysis but also for a full range of epigenetic profiling services. We support investigations into other key histone PTMs—including methylation, acetylation, phosphorylation, ubiquitination—with the same level of precision and dedication. From single-modification studies to integrated multi-omics projects, we provide comprehensive, tailored solutions to help you uncover the mechanisms driving health and disease. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.
Reference
1. Stein R A, Riber L. Epigenetic effects of short-chain fatty acids from the large intestine on host cells[J]. Microlife, 2023, 4: uqad032.
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