Histone Lysine Acylation Analysis

Histone lysine acylation is a dynamic and rapidly developing frontier in epigenetics, where metabolic signals are directly imprinted on chromatin, thereby regulating gene expression and cell fate. To comprehensively understand this complex signaling pathway, CD BioSciences offers authoritative histone lysine acylation analysis services, helping you delve into this crucial link between cellular metabolism and gene regulation.

Overview of Histone Lysine Acylation

Histone lysine acylation represents a dynamic and rapidly expanding layer of epigenetic regulation, extending far beyond the well‑characterized acetylation. This class of post‑translational modifications includes diverse acyl groups—such as propionyl, butyryl, crotonyl, succinyl, and malonyl—that are derived from cellular metabolites and enzymatically transferred to specific lysine residues on histone tails. These modifications directly alter chromatin structure and function, acting as crucial chemical sensors that link cellular metabolic states to gene expression programs. By recruiting distinct "reader" proteins and modulating transcriptional activity, histone acylation plays pivotal roles in development, metabolism, immune response, and disease pathogenesis, making it a key frontier in understanding the interface between metabolism and epigenetics.

Fig.1 Profiling lysine propionylation in Synechocystis sp. PCC 6803. (Yang M, et al., 2019)

Our Services

Understanding the precise patterns of these dynamic modifications is crucial for deciphering how metabolic signals are integrated into the epigenetic code to regulate gene expression in both healthy and disease states. CD BioSciences offers comprehensive and professional histone lysine acylation analysis services, from highly sensitive mass spectrometry and precise quantification to integrated bioinformatics interpretation, helping researchers uncover new biological insights and advance the development of therapeutics.

Comprehensive Solution for Histone Lysine Acylation Analysis

Our service leverages a powerful synergy of cutting-edge high-resolution mass spectrometry platforms and a proprietary bioinformatics pipeline to ensure unparalleled sensitivity, accuracy, and depth of analysis.

Analyzable Modification Types

We offer precise analysis for a wide spectrum of histone lysine acylations, including but not limited to:

1. Propionylation (Kpr)
2. Butyrylation (Kbu)
3. Crotonylation (Kcr)

1. Succinylation (Ksucc)
2. Malonylation (Kma)
3. 2-Hydroxyisobutyrylation (Khib)

1. β-Hydroxybutyrylation (Kbhb)
2. Glutarylation (Kglu)
3. Lactylation (Klac)

Core Service Modules

1. Panoramic Screening & Discovery: Utilize unbiased, global profiling to identify and map both novel and known histone lysine acylation marks across your samples.
2. Targeted Precision Quantification: Achieve highly sensitive and reproducible absolute or relative quantification of specific, pre-defined acylation marks to validate hypotheses across experimental conditions.
3. Multi-Dimensional Integrated Analysis: Uncover functional insights and mechanistic relationships by integrating acylation data with complementary omics datasets, such as transcriptomics or metabolomics.

Workflow of Histone Lysine Acylation Analysis Service

Supported Sample Types

1. Cell Lines & Primary Cells: Including adherent, suspension, and difficult-to-lyse cells.
2. Animal & Human Tissues: Such as fresh, snap-frozen, or FFPE tissue sections.
3. Immunoprecipitated (IP) Samples: Including histone- or specific modification-enriched samples.
4. Purified Histone Fractions: Isolated via acid extraction or other purification methods.
5. Other Custom Matrices: Please consult our technical team for specialized sample types.

Why Choose Us?

1. Expert-Led Science: Benefit from a project team led by PhD-level scientists with dual expertise in mass spectrometry technology and epigenetics.
2. Proven Experience & Infrastructure: Leverage our extensive project portfolio and a proprietary, in-house spectral library for histone acylation to ensure superior identification accuracy and reliability.
3. End-to-End Quality Assurance: Rely on a seamless, standardized workflow with stringent quality control checkpoints implemented from sample intake to final data delivery.
4. Actionable, Publication-Ready Insights: Move beyond data lists to receive deeply analyzed results with clear biological interpretation, designed to support high-impact publications and grant applications.
5. Fully Customizable Solutions: Access flexible service tiers and tailored project designs that align precisely with your specific scientific objectives and budgetary requirements.

At CD BioSciences, our expertise in histone lysine acylation analysis is just one facet of our comprehensive epigenetic research platform. We are your dedicated partner in deciphering the complex language of chromatin. Beyond acylations, we provide equally rigorous, end-to-end analytical services for a full spectrum of histone PTMs, including methylation, acetylation, phosphorylation, ubiquitination, and beyond. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

1.Yang M, Huang H, Ge F. Lysine propionylation is a widespread post-translational modification involved in regulation of photosynthesis and metabolism in cyanobacteria[J]. International Journal of Molecular Sciences, 2019, 20(19): 4792.