ITC Binding Assay Service

CD BioSciences provides expert isothermal titration calorimetry (ITC) binding assay services for comprehensive thermodynamic profiling of biomolecular interactions. Our label-free approach directly measures binding affinity, stoichiometry, and enthalpy/entropy changes, delivering critical insights for epigenetic mechanism research and drug discovery.

Overview of Isothermal Titration Calorimetry (ITC)

ITC is a label-free technique that directly measures the heat released or absorbed during a binding event. In an experiment, the instrument titrates one binding partner (the ligand) into a solution containing the other (the macromolecule). Each injection produces a heat signal, which is plotted to generate a binding isotherm. Analysis of this isotherm provides a complete thermodynamic profile by simultaneously determining the affinity (KD), stoichiometry (n), enthalpy change (ΔH), and entropy change (ΔS). This ability to directly elucidate the driving forces of biomolecular interactions makes ITC a fundamental tool for optimizing interactions in areas such as epigenetic research and rational drug design.

Fig.1 Applications of isothermal titration calorimetry in studying biomimetic nanocarriers. (Guerrero M, et al., 2025)

Our Services

Leveraging state-of-the-art microcalorimetry platforms, CD BioSciences' ITC service is designed for precision and reliability. We specialize in characterizing the thermodynamic signatures of diverse molecular interactions, providing a deep understanding of binding energetics that is essential for guiding research and development decisions.

We offer comprehensive ITC analysis tailored to your research objectives:

1. Full Thermodynamic Profiling: Simultaneous determination of binding affinity (KD), enthalpy change (ΔH), entropy change (ΔS), and stoichiometry (n).
2. Binding Constant Determination: Accurate measurement of association constants across a wide range (Ka ~ 10³ to 10⁸ M⁻¹).
3. Interaction Types: Ideal for studying Protein-Protein, Protein-Small Molecule, Protein-DNA/RNA, and other biomolecular interactions.

Service Workflow

Sample Requirements

1. Macromolecule (Cell): High purity (>95%), typical concentration range of 10-100 µM.
2. Ligand (Syringe): High purity (>95%), concentration typically 10-20 times that of the macromolecule.
3. Buffer Compatibility: Requires careful matching of buffers to avoid heat of dilution artifacts.

Deliverables

Upon project completion, you will receive:

1. A detailed final report including all experimental conditions.
2. Raw titration data and the fitted binding isotherm.
3. Calculated thermodynamic parameters (KD, n, ΔH, ΔS, ΔG).
4. Professional interpretation of the results.

CD BioSciences' Technology Advantages

1. Label-Free & Solution-Based: Interactions are studied without immobilization or labeling, under near-physiological conditions.
2. Complete Thermodynamic Data: Provides a full picture of the interaction forces (enthalpy-driven vs. entropy-driven).
3. High Information Content: A single experiment yields affinity, stoichiometry, and thermodynamics.
4. Advanced Instrumentation: Our services utilize high-sensitivity microcalorimeters (e.g., Malvern MicroCal PEAQ-ITC) to ensure data accuracy.

Frequently Asked Questions (FAQs)

CD BioSciences' ITC binding assay service delivers robust, high-quality thermodynamic data to accelerate your epigenetic research. Leveraging cutting-edge platforms and rigorous validation, we ensure reliable results for your projects. Contact us today for a free project consultation and a detailed quote. We are here to help you gain deep, mechanistic insights.

Reference

1. Guerrero M, Braden C, Bao Y. Applications of Isothermal Titration Calorimetry in Studying Biomimetic Nanocarriers[J]. Biomolecules, 2025, 15(10): 1349.