A primary biomarker of oxidative DNA damage, 8-oxoguanine (8-oxoG) is a critical lesion linked to genomic instability, aging, and the pathogenesis of numerous diseases. CD BioSciences provides accurate and sensitive quantification and detection services for 8-oxoG, enabling researchers to assess cellular oxidative stress, DNA repair capacity, and the impact of environmental or therapeutic agents.

Introduction to 8-Oxoguanine (8-oxoG)

8-Oxoguanine (8-oxoG) is one of the most abundant and well-studied lesions resulting from the oxidation of guanine bases by reactive oxygen species (ROS). This mutagenic modification alters the base-pairing properties of guanine, frequently causing it to mispair with adenine during DNA replication. This leads to a characteristic G:C to T:A transversion mutation if left unrepaired, making 8-oxoG a major endogenous source of genomic instability. Cells have evolved a dedicated base excision repair (BER) pathway, initiated by enzymes such as OGG1, to specifically recognize and excise 8-oxoG.

Consequently, the steady-state level of 8-oxoG in genomic DNA reflects a dynamic balance between the rate of oxidative insult and the efficiency of cellular repair mechanisms. Its measurement is therefore a pivotal biomarker for evaluating oxidative stress in conditions such as aging, neurodegenerative disorders (e.g., Alzheimer's, Parkinson's), cancer, metabolic diseases, and in response to environmental toxins or radiation. Analyzing 8-oxoG provides direct insight into DNA damage burden, repair fidelity, and their contribution to disease etiology.

Fig.1 Schematic representation of guanine (G) oxidation to 8-oxoguanine (8-oxoG). (Chiorcea-Paquim A M, Oliveira-Brett A M., 2021)

Our Services

At CD BioSciences, we offer comprehensive solutions for the specific and quantitative analysis of 8-oxoguanine (8-oxoG). Understanding that accurate measurement is key to interpreting biological and clinical outcomes, we employ validated gold-standard and high-throughput methodologies. Our services empower your research by providing reliable data to link oxidative DNA damage to functional consequences in models of disease, toxicology, and aging.

Comprehensive 8-oxoG Analysis Solutions

Our platform delivers precise and scalable quantification of oxidative DNA damage, supporting research from detailed mechanistic studies to high-throughput biomarker screening.

1. Global DNA Modification Quantification by LC-MS/MS: Provides absolute quantification of 8-oxoG with gold-standard accuracy and specificity for definitive biological measurement.
2. Global DNA Modification Quantification by ELISA: Enables cost-effective, high-throughput relative quantification, ideal for screening large sample cohorts and comparative studies.

Workflow of DNA 8-oxoG Analysis Service

Following a standardized and contamination-controlled workflow, CD BioSciences' 8-oxoG analysis service prevents artifactual oxidation during sample processing and ensures the generation of reliable, reproducible data for both absolute (LC-MS/MS) and relative (ELISA) quantification.

Initial Consultation and Assay Selection

Our specialists work with you to define the study objective and select the optimal assay (LC-MS/MS for absolute quantification or ELISA for high-throughput screening) based on required precision, sample number, and project scope.

Controlled Sample Collection and DNA Isolation

Samples are processed using optimized protocols that minimize ex vivo oxidation. DNA is isolated under antioxidant-protected conditions (e.g., using chelating agents and radical scavengers) and quantified with fluorometric methods to ensure accurate normalization.

Standardized DNA Hydrolysis and Derivatization

For LC-MS/MS, isolated DNA undergoes enzymatic digestion to deoxyribonucleosides under controlled conditions. For ELISA, DNA is denatured to expose the 8-oxodG epitope. Both processes include the preparation of calibration standards and quality control samples within each batch.

High-Sensitivity Detection and Data Acquisition

Processed samples are analyzed on state-of-the-art instrumentation. LC-MS/MS analysis is performed using triple-quadrupole mass spectrometry in MRM mode for maximum specificity. ELISA plates are read on a calibrated spectrophotometric plate reader.

Rigorous Data Processing and Normalization

Raw data is processed against standard curves. For LC-MS/MS, 8-oxodG levels are normalized to the total deoxyguanosine (dG) content in the same sample, expressed as the 8-oxodG/dG ratio. For ELISA, results are normalized to total DNA input.

Comprehensive Quality Control and Statistical Analysis

We perform batch-level QC using internal controls and report key metrics (e.g., CV%, recovery rates). Data is then analyzed with appropriate statistical methods to compare oxidative damage levels across experimental groups.

Detailed Reporting and Biological Interpretation

We deliver a final report containing all quantified results, statistical analyses, methodology details, and a contextual interpretation of the findings in relation to oxidative stress biology and your specific research model.

Our Advantages

1. Gold-Standard Precision: Our LC-MS/MS service provides definitive absolute quantification, essential for biomarker validation and mechanistic studies.
2. High-Throughput Efficiency: Our ELISA platform enables rapid, cost-effective screening of large sample cohorts, ideal for population studies and toxicity screening.
3. Contamination-Controlled Protocols: Stringent workflows prevent ex vivo oxidation, ensuring measured 8-oxoG levels reflect true biological damage.
4. Comprehensive Data Normalization: We ensure biological relevance by normalizing LC-MS/MS data to total dG content and ELISA data to total DNA input.
5. Dedicated Oxidative Stress Expertise: Our team provides contextual data interpretation, linking quantitative results to biological and clinical implications.

As a specialized provider of epigenetics services, CD BioSciences is committed to providing comprehensive support for your genome integrity and epigenetics research. Beyond 8-oxoG and oxidative damage, we offer a full suite of services for the analysis of DNA methylation (5mC, 5hmC, 5fC, 5caC) and other base modifications (6mA, 4mC). From environmental toxicology and aging research to cancer biology and neurogeneration, our customizable workflows are designed to provide the specific insights your project requires. Contact us to develop a targeted analysis plan.

Reference
1. Chiorcea-Paquim A M, Oliveira-Brett A M. Nanostructured material–based electrochemical sensing of oxidative DNA damage biomarkers 8-oxoguanine and 8-oxodeoxyguanosine: A comprehensive review[J]. Microchimica Acta, 2021, 188(2): 58.