Nanopore Sequencing Service

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Conventional short-read sequencing struggles to resolve complex genomic regions, structural variations, and the native landscape of epigenetic modifications. Nanopore sequencing overcomes these limitations by delivering ultra-long reads, real-time data, and direct detection of DNA/RNA modifications in a single assay. At CD BioSciences, we transform this revolutionary technology into reliable, end-to-end solutions, guiding you from experimental design to advanced analysis to unlock complete genomic and epigenomic insights.

Overview of Nanopore Sequencing

Nanopore sequencing is a revolutionary third-generation technology that determines the sequence of DNA or RNA molecules in real time by measuring changes in electrical current as nucleic acids pass through a protein nanopore. In this direct, label-free method, each individual nucleotide (or chemical modification) causes a characteristic disruption in the ionic current as it traverses the pore, enabling both ultra-long reads—often spanning tens to hundreds of kilobases—and the simultaneous detection of base modifications, such as 5mC and 6mA, without the need for bisulfite conversion or other pre-treatment.

The basic principle of nanopore sequencing.

Fig.1 Principle of nanopore sequencing. (Chen J, Xu F., 2023)

Advantages of Nanopore Sequencing

Nanopore sequencing offers a paradigm shift in genomic analysis by delivering ultra-long reads, real-time data stream, and the direct detection of nucleotide modifications in a single, streamlined workflow. This unique combination of features overcomes fundamental limitations of short-read technologies, enabling researchers to tackle previously intractable genomic complexities with unprecedented clarity and speed.

Ultra-Long Reads: Enables sequencing of DNA molecules spanning tens to hundreds of kilobases, resolving complex genomic regions and simplifying assembly.
Real-Time Data Generation: Provides immediate access to sequencing data as it is produced, allowing for rapid analysis and adaptive, on-the-fly decision-making.
Direct Epigenetic Detection: Natively identifies base modifications (e.g., 5mC, 6mA) directly from the sequencing signal without chemical conversion or special treatment.
Portable and Scalable Platforms: Offers flexibility from pocket-sized, field-ready devices to high-throughput benchtop systems within a unified technology ecosystem.
Streamlined and Direct Workflow: Features simple, rapid library preparation, often without PCR, and can sequence native, unamplified DNA/RNA molecules.

Our Services

CD BioSciences provides comprehensive, end-to-end nanopore sequencing services, leveraging the unique advantages of ultra-long reads and real-time analysis to deliver unparalleled insights across a spectrum of complex genomic applications. Our expert service supports critical research needs, including high-quality genome assembly, precise structural variant detection, full-length transcriptome sequencing (Iso-seq), direct pathogen identification and metagenomic analysis, and native epigenetic profiling.

Customized Nanopore Sequencing Solutions

To address the specific challenges presented by complex genomes and dynamic biological systems, CD BioSciences offers a suite of customized nanopore sequencing solutions. Leveraging the unique capabilities of ultra-long reads and real-time, native sequencing, we tailor our expertise and workflows to deliver precise insights across five core application areas.

Service	Application	Description
Direct Epigenetic Detection Service	Simultaneously acquire sequence information and base modification profiles (e.g., 5mC, 5hmC, 6mA, 4mC) in a single assay.	Performs sequencing on native DNA, using specialized analysis tools to directly detect epigenetic modifications from the raw signal data, preserving the native molecular state.
De novo Genome/Metagenome Assembly Service	Obtain high-quality, complete reference genomes or metagenome assemblies.	Utilizes ultra-long reads for superior contiguity, often combined with short-read polishing (hybrid assembly) to generate highly continuous, accurate scaffolds.
Structural Variant & Complex Region Analysis Service	Precisely detect large-scale insertions, deletions, translocations, inversions, and tandem repeats.	Employs long-read whole-genome sequencing to span repetitive regions, enabling the precise mapping of variant breakpoints and resolution of complex genomic architectures.
Full-Length Transcriptome Sequencing (Iso-Seq) Service	Directly obtain complete, end-to-end mRNA sequences to accurately identify isoforms, alternative splicing, and gene fusions.	Sequences full-length cDNA molecules without assembly, providing a comprehensive and quantitative catalog of transcript isoforms and their structures.
Rapid Pathogen Identification & Typing Service	Achieve fast (within hours) identification of pathogens, along with antibiotic resistance and virulence profiling, from clinical or environmental samples.	Leverages real-time sequencing with rapid library kits and analysis workflows for immediate, on-site detection and characterization of microbial communities.

Workflow of Nanopore Sequencing Service

Our Advantages

Expertise in Long-Read Applications: Our deep experience in nanopore sequencing ensures optimal experimental design and execution for complex applications like genome assembly, full-length transcriptomics, and direct epigenetics.
Broad Sample Compatibility: We are proficient in handling diverse and challenging sample types, including human/animal tissues, blood, plants, microbes, FFPE samples, and cfDNA, with specialized protocols for each.
End-to-End Native Sequencing Workflow: We specialize in native DNA/RNA library preparation, preserving base modifications and avoiding PCR bias to deliver biologically accurate long-read data.
Integrated Bioinformatics Pipeline: We provide specialized analysis using the latest long-read tools (e.g., Flye, Sniffles, FLAIR) tailored to each application, transforming raw data into interpretable biological insights.

Nanopore sequencing represents a transformative leap in genomic analysis, and at CD BioSciences, we bridge the gap between this powerful technology and your research goals. Our comprehensive service portfolio is built on a foundation of deep expertise, tailored workflows, and advanced bioinformatics. We simplify the complexity of long-read sequencing, empowering you to generate complete, accurate, and biologically insightful data. Contact us today to discuss how our customized nanopore sequencing solutions can accelerate your next discovery.

Reference 1. Chen J, Xu F. Application of nanopore sequencing in the diagnosis and treatment of pulmonary infections[J]. Molecular Diagnosis & Therapy, 2023, 27(6): 685-701.