DNA methylation cannot be directly investigated using conventional DNA amplification because methylation markers cannot be maintained during this period. Bisulfite conversion is one of the most widely used methods to convert sample with DNA methylation markers into appropriate amplification and downstream analysis templates. EpigenHub is capable to provide our customers with superior bisulfite conversion service, ensuring high-quality templates of DNA methylation analysis for downstream applications based on bisulfite conversion.

Basic Principle of Bisulfite Conversion

The principle of bisulfite conversion is based on the chemical conversion of unmethylated cytosine to uracil, while methylated cytosine is exempt from this conversion, allowing DNA methylation to be determined at the single-base resolution. Bisulfite conversion is a process in which DNA is denatured (made into a single strand) and treated with sodium bisulfite, resulting in deamination of unmethylated cytosine to uracil, while methylated cytosine (5-mC and 5-hmC) remains unchanged.

Fig 1. The principle of bisulfite conversion

Application of Bisulfite Conversion

1. Bisulfite sequencing PCR
2. Methylation-specific PCR
3. Pyrosequencing
4. High resolution melting (HRM) analysis
5. Methylation-sensitive single-nucleotide primer extension
6. Whole-genome bisulfite sequencing
7. Reduced representation bisulfite sequencing
8. DNA methylation microarrays

Our Advantages

1. Accept a wide range of sample types (purified DNA, whole blood, cells, tissues, formalin fixed paraffin-embedded tissues, etc.)
2. Ensure high recovery of high quality DNA and minimize degradation
3. Provide greater than 99% conversion efficiency
4. Short experimental period

Workflow of Bisulfite Conversion at EpigenHub
EpigenHub strictly controls each specific experimental step in the bisulfite conversion procedure to ensure accurate conversion of unmethylated cytosine to uracil in the obtained DNA.

Fig 2. Workflow of bisulfite conversion at EpigenHub

Please feel free to contact us if you would like to know more about bisulfite conversion service. At EpigenHub, we not only provide high-quality bisulfite conversion service, but also offer a range of downstream DNA methylation analysis services. Additionally, our experts can also provide and help design the best solution according to your specific requirements.