5-Methylcytosine (5mC), as the most stable and core DNA methylation modification, is the biological code for decoding developmental programming, disease mechanisms, and environmental responses. From whole-genome mapping to single-base precision, from tissue samples to single-cell resolution, CD BioSciences provides comprehensive, accurate, and reliable 5mC analysis solutions, helping you delve into the mysteries of the epigenetic world and transform data into real scientific discoveries.

Overview of 5-Methylcytosine (5mC)

5-Methylcytosine (5mC) is a fundamental epigenetic modification involving the addition of a methyl group to the fifth carbon of cytosine bases, primarily within CpG dinucleotides in eukaryotic DNA. As the most prevalent and stable DNA modification, it serves as a critical regulatory mechanism for gene expression, typically associated with transcriptional repression when located in promoter regions. 5mC plays essential roles in genomic imprinting, X-chromosome inactivation, silencing of transposable elements, and cellular differentiation during development. Its dynamic patterns are crucial for normal physiological processes, while dysregulation is implicated in various diseases, particularly cancer, where global hypomethylation and site-specific hypermethylation of tumor suppressor genes are hallmarks. In research and clinical applications, 5mC analysis enables biomarker discovery for early disease detection, prognostic assessment, and therapeutic monitoring. It also advances understanding of aging, environmental adaptation, and developmental biology through techniques ranging from locus-specific methylation analysis to genome-wide profiling, making it a cornerstone of modern epigenetics studies.

Fig.1 Cytosine methylation and oxidation cycle. (Turpin M, Salbert G., 2022)

Our Services

CD BioSciences provides comprehensive, end-to-end DNA 5-methylcytosine (5mC) analysis services, empowering your epigenetic research with precision and depth. We offer a complete suite of validated technologies, from gold-standard bisulfite sequencing for single-base resolution to high-throughput arrays for population-scale studies. Our expert team manages your project from design to delivery, selecting the optimal platform and providing deep bioinformatic analysis. We transform your samples into reliable, publication-ready results, delivering clear insights into methylation patterns for your research in development, disease, or biomarker discovery.

Comprehensive 5mC Analysis Solutions

At CD BioSciences, our DNA 5-methylcytosine (5mC) analysis is powered by a versatile and robust technology platform, designed to deliver accurate, reliable, and scalable data tailored to your specific research goals and sample type. We offer a comprehensive portfolio of gold-standard and cutting-edge methodologies.

1. Global DNA Modification Quantification by LC-MS/MS
2. Global DNA Modification Quantification by ELISA
3. Whole Genome Bisulfite Sequencing (WGBS) Service
4. Reduced Representation Bisulfite Sequencing (RRBS) Service
5. Targeted Bisulfite Sequencing (Capture-based) Service
6. Single-Cell Bisulfite Sequencing (scBS-seq/scRRBS) Service
7. Bisulfite Sequencing PCR (BSP) Service
8. Methylation-Specific PCR (MSP) Service
9. Pyrosequencing Service

1. Methylation-Sensitive High-Resolution Melting (MS-HRM) Analysis Service
2. Bisulfite Conversion Service
3. Bisulfite Library Preparation Service
4. DNA Methylation Microarray Service
5. Methylation-sensitive Restriction Enzyme (MRE) Sequencing Service
6. Methyl-CpG-Binding Domain (MBD) Sequencing Service
7. Oxidative Bisulfite Sequencing (oxBS-seq) Service
8. DNA Immunoprecipitation (DIP) Sequencing Service
9. Nanopore Sequencing Service

Workflow of DNA 5mC Analysis Service

Leveraging our advanced technology platform and expert scientific team, CD BioSciences has established a standardized end-to-end workflow from sample to insight. We ensure precision and reproducibility at every step through rigorous quality control, delivering high-quality, publication-ready 5mC methylation data through transparent and efficient collaboration.

Our Advantages

Comprehensive Technology Portfolio

We offer both gold-standard and next-generation sequencing platforms, from WGBS and RRBS to targeted analysis and high-throughput arrays, ensuring the optimal solution for every research need.

End-to-End Analysis Depth

Our service extends from precise methylation detection to advanced bioinformatics interpretation, delivering not just data, but clear biological insights and actionable conclusions.

Dedicated Scientific Partnership

We provide full-project support, from experimental design and execution to final reporting, guided by our experienced team of epigenetics specialists throughout your project lifecycle.

Broad Sample & Species Compatibility

Our optimized protocols support a wide range of sample types, including tissues, cells, blood, and FFPE samples, across multiple species, maximizing the applicability of your research.

Commitment to Quality & Reliability

Every step of our workflow is governed by stringent quality controls, ensuring highly reproducible, publication-ready results you can trust.

At CD BioSciences, we provide a comprehensive and highly customizable DNA modification analysis services. While specializing in 5mC mapping, our expertise extends to the complete epigenetic landscape, including 5hmC, 5fC, 5caC, 6mA, 4mC, and 8-oxoG analysis. Whether your focus is on gene regulation, disease mechanisms, or biomarker discovery, we tailor our validated workflows to your specific research goals—delivering precise, reliable data to drive your discoveries forward. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference
1. Turpin M, Salbert G. 5-methylcytosine turnover: Mechanisms and therapeutic implications in cancer[J]. Frontiers in Molecular Biosciences, 2022, 9: 976862.