Chromatin Accessibility & Profiling Service

Chromatin accessibility and higher-order genome organization shape how regulatory elements (promoters, enhancers, insulators) control transcription. By profiling open chromatin, DNA–protein interactions, nucleosome positioning, and chromatin looping/contacts, researchers can connect epigenetic mechanisms to gene expression, cell state transitions, and disease phenotypes. CD BioSciences' chromatin accessibility & profiling service provides an integrated, preclinical research workflow that supports the full spectrum of chromatin studies—from rapid accessibility screening to high-resolution chromatin occupancy mapping and 3D genome conformation analysis.

Applications of Chromatin Accessibility & Profiling

Chromatin accessibility and higher-order genome organization influence how regulatory elements control transcription across cell states and disease contexts.

1. Regulatory element discovery – Identify promoters, enhancers, silencers, and insulators driving gene programs.
2. Mechanism of action (MoA) studies – Reveal how TF binding, histone marks, and chromatin remodeling change under perturbations.
3. Drug response and perturbation analysis – Compare chromatin rewiring across treatments, doses, and time points.
4. Cell fate and differentiation research – Track epigenetic reprogramming during lineage commitment or induction.
5. Cancer epigenetics – Profile super-enhancers, oncogene regulation, and tumor-associated chromatin states.
6. 3D genome mechanism studies – Map enhancer–promoter looping, domain boundary shifts, and topology changes.

Fig.1 ATAC-seq probes genome-wide chromatin accessibility using hyperactive Tn5 transposase. (Sinha, S., et al., 2021)

Our Services

CD BioSciences provides an integrated portfolio of chromatin accessibility & profiling services spanning three complementary dimensions of epigenetic regulation: (1) chromatin accessibility and nucleosome landscape, (2) protein-centric chromatin occupancy and complex capture, and (3) 3D genome architecture and chromatin contacts. You may choose a single assay for focused questions or combine multiple modules into a discovery-to-validation workflow.

Supported Sample Types

1. Cultured Cells
2. Primary Cells
3. Fresh-Frozen Tissues
4. FFPE Tissue Sections

1. Low-Input and Micro-Dissected Samples
2. Time-Course, Drug-Treated, or Perturbation Samples
3. Cell Differentiation or Induction Models
4. Other Types (Please Inquire)

Our Advantages

1. Modular, Bundle-Friendly Design: Build a rational pipeline (e.g., ATAC-seq → CUT&Tag → HiChIP/4C; or MNase-seq → Histone ChIP-seq → Hi-C) based on your question.
2. Low-Input Options Available: CUT&RUN/CUT&Tag and targeted 3C variants (4C/5C) support projects with limited material (assay-specific feasibility assessed).
3. Quality-First Execution: Assay-specific QC checkpoints across nuclei prep, fragmentation/tagmentation, enrichment, and sequencing metrics.
4. Interpretation-Ready Outputs: Peak files, accessibility tracks, nucleosome occupancy, interaction matrices, loop calls, and annotated reports designed for downstream biology.

Build a clear, mechanistic view of gene regulation by combining chromatin accessibility, occupancy, and 3D genome architecture profiling. CD BioSciences' chromatin accessibility & profiling service supports single-assay projects and integrated multi-assay pipelines—contact us to discuss your sample type, biological question, and the best-fit assay strategy for your study.

Reference

1. Sinha, S., et al. (2021). Profiling Chromatin Accessibility at Single-cell Resolution. Genomics, proteomics & bioinformatics, 19(2), 172–190.