| Cat.No.: | PE-1112 |
| Product Name: | Recombinant Human MGMT, His-tagged |
| Product Overview: | Recombinant full length human MGMT was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. |
| Description: | MGMT or O-6-methylguanine-DNA methyltransferase is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. The methylation of the MGMT promoter in gliomas is a useful predictor of the responsiveness of the tumors to alkylating agents. The clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide. Methylation of the MGMT promoter in tumors is associated with longer survival in glioblastoma patients. |
| Applications: | Western Blot |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | ~25 kDa |
| Purity: | >95% |
| Species: | Human |
| Formulation: | Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
| Tag: | His |
| Expression System: | Sf9 Insect Cells |
| Protein Length: | Full length |
| Concentrations: | 0.1 μg/μ |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ] |
| Gene ID NCBI: | 4255 |
| Synonyms: | MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; |
| mRNA Refseq: | NM_002412 |
| Protein Refseq: | NP_002403 |
| MIM: | 156569 |
| UniProt ID: | P16455 |
| Chromosome Location: | 10q26 |
| Function: | DNA binding; DNA-methyltransferase activity; calcium ion binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity; transferase activity; |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0142 | Recombinant Human MGMT Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0164 | Lomeguatrib | Inquiry |
| ◆ Antibodies | ||
| EAb-0230 | O6-Methylguanine-DNA methyltransferase Polyclonal Antibody | Inquiry |
| EAb-0351 | MGMT Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0394 | Human MGMT Knockout Cell Line 2bp deletion | Inquiry |
| Related Gene / Proteins | |||
| MGMT | |||
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