| Cat.No.: | PE-1111 |
| Product Name: | Recombinant Human MGMT protein, His-tagged |
| Product Overview: | Recombinant Human MGMT(1-207aa) fused with His tag at N-terminal was expressed in E. coli. |
| Description: |
O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT ex |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher) |
| Purity: | > 95% by SDS - PAGE |
| Species: | Human |
| Formulation: | Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol |
| Tag: | His |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYFHQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSGGLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN |
| Endotoxin: | < 1.0 EU per 1 microgram of protein (determined by LAL method) |
| Expression System: | E. coli |
| Concentrations: | 0.5 mg/ml (determined by Bradford assay) |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ] |
| Gene ID NCBI: | 4255 |
| Official Symbol: | MGMT |
| Synonyms: | MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; |
| mRNA Refseq: | NM_002412 |
| Protein Refseq: | NP_002403 |
| MIM: | 156569 |
| UniProt ID: | P16455 |
| Chromosome Location: | 10q26 |
| Function: | DNA binding; DNA-methyltransferase activity; calcium ion binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity; transferase activity; |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0142 | Recombinant Human MGMT Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0164 | Lomeguatrib | Inquiry |
| ◆ Antibodies | ||
| EAb-0230 | O6-Methylguanine-DNA methyltransferase Polyclonal Antibody | Inquiry |
| EAb-0351 | MGMT Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0394 | Human MGMT Knockout Cell Line 2bp deletion | Inquiry |
| Related Gene / Proteins | |||
| MGMT | |||
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