| Cat.No.: | PE-1084 |
| Product Overview: | Recombinant Human DOT1L(1-435 aa) fused with GST tag at N-terminal was expressed in Insect cells. |
| Description: | DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones. DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis. |
| Applications: | Methyltransferase Assay |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 78 kDa |
| Purity: | >90% |
| Species: | Human |
| Formulation: | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Tag: | GST |
| Activity: | 14 nmol/min/mg |
| Expression System: | Insect Cells |
| Protein Length: | 1-435 aa |
| Concentrations: | 0.1μg/μl |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | DOT1L DOT1-like, histone H3 methyltransferase (S. cerevisiae) [ Homo sapiens ] |
| Gene ID NCBI: | 84444 |
| Official Symbol: | DOT1L |
| Synonyms: | DOT1L; DOT1-like, histone H3 methyltransferase (S. cerevisiae); histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1; histone methyltransferase DOT1L; KIAA1814; KMT4; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone H3-K7 |
| mRNA Refseq: | NM_032482 |
| Protein Refseq: | NP_115871 |
| MIM: | 607375 |
| UniProt ID: | Q8TEK3 |
| Chromosome Location: | 19p13.3 |
| Function: | DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transcription factor binding; transferase activity; |
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