| Cat.No.: | PE-0685 |
| Product Name: | Recombinant Human SP1, HA & Flag-tagged |
| Product Overview: | Recombinant Human SP1, fused to a HA- and a FLAG-tag, was expressed in insect cells (Schneider SL2 cells). |
| Description: | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. |
| Applications: | EMSA promoter characterization, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control). |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Species: | Human |
| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.9, containing 420mM KCl, 0.1% NP-40, 12.5mM MgCl2 and 20% glycerol. |
| Tag: | HA & Flag |
| Activity: | 0.5BFU/μl (band forming units). 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide. |
| Expression System: | Insect Cells |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | SP1 Sp1 transcription factor [ Homo sapiens (human) ] |
| Gene ID NCBI: | 6667 |
| Synonyms: | SP1; Sp1 transcription factor; Sp1 transcription factor; specificity protein 1; TSFP1; Transcription factor Sp1 |
| mRNA Refseq: | NM_003109 |
| Protein Refseq: | NP_003100 |
| MIM: | 189906 |
| UniProt ID: | P08047 |
| Chromosome Location: | 12q13.1 |
| Function: | RNA polymerase II transcription factor activity; double-stranded DNA binding; histone deacetylase binding; metal ion binding; promoter binding; protein C-terminus binding; protein homodimerization activity; transcription activator activity; transcription factor activity; zinc ion binding |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0089 | Recombinant Human SP2 Cell Lysate | Inquiry |
| ◆ Cell Lines | ||
| CL-0161 | Human SP1 Knockout Cell Line 2bp deletion | Inquiry |
| CL-0169 | Human SP140L Knockout Cell Line | Inquiry |
| CL-0170 | Human SP100 Knockout Cell Line 20bp deletion | Inquiry |
| CL-0171 | Human SP110 Knockout Cell Line 7bp deletion | Inquiry |
| Related Gene / Proteins | |||
| SP1 | SP100 | SP110 | SP140 |
| SP140L | SP2 | SP3 | SP6 |
| SPDL1 | SPF30 | SPIN1 | SPOP |
| SPT16 | SPT3 | Spt6 | SPTY2D1 |
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