| Cat.No.: | EAb-2305 |
| Antibody Type: | Monoclonal |
| Immunogen: | This antibody was raised against 5-methyl-cytosine-adenosine di-nucleoside conjugated to KLH and recognized 5-mCpA. |
| Host: | Mouse |
| Isotype: | IgG1 |
| Purification: | Protein A Chromatography |
| Appearance: | Liquid |
| Formulation: | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| Applications: | Dot Blot (DB): 1 µg/ml^Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Not Species Specific |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Scientific Background: | DNA methylation is important for regulation of transcription, and in processes including imprinting, gene silencing and cancer development. Methylation occurs predominantly at cytosine within the dinucleotide CpG (meCpG), which is frequently found in promoter regions near transcription start sites, as well as in promoters for functional non-coding RNAs. However, methylation at CpG dinucleotides makes them susceptible to both spontaneous deamination and enzyme-mediated deamination, resulting in thymine substitution (T/G mismatch) and the formation of a CpA dinucleotide in the opposite strand. Therefore, there is a strong correlation of CpG dinucleotide depletion or “suppression” with an observed increase in TpG/CpA dinucleotides.In mammals, there are certain cell types in which significant levels of methylation at CpA, (meCpA), CpT (meCpT), CpC (meCpC) is also observed, including embryonic stem cells, oocytes, primordial germ cells and neurons. Early observations suggest that meCpA, in particular, has different nuclear distribution than meCpG, and that meCpA may associate with active transcription rather than suppression. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0001 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0209 | 5-Hydroxymethyluridine Polyclonal Antibody | Inquiry |
| EAb-0226 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0254 | 5-Methylcytosine (5-mC) Monoclonal Antibody (RM231) | Inquiry |
| EAb-0255 | 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| 5-caC | 5-fC | 5-hmC | 5-Hydroxymethyluridine |
| 5-mC | 5-mCpA | ||
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