| Cat.No.: | EAb-0544 |
| Product Overview: | Anti-5-hydroxymethylcytosine (MOUSE) Monoclonal Antibody |
| Antibody Type: | Monoclonal |
| Immunogen: | Produced in mice by repeated immunization with 5-hydroxymethlcytosine. |
| Host: | Mouse |
| Isotype: | IgG1 |
| Purification: | Purified by Protein A chromatography. |
| Appearance: | Liquid |
| Formulation: | 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 |
| Applications: | Dot Blotting, ELISA, Hydroxymethylated DNA Immunoprecipitation Sequencing and Immunofluorescence |
| Recommended Dilutions/Conditions: |
ELISA: 1:500; hMeDIP: 2.5μg per IP; Dot Blot: 2 μg/ml Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Cross Reactivity: | Human, Mouse, Other (wide range) |
| Shipping: | Dry Ice |
| Storage: | -20°C |
| Storage Buffer: | 0.05% (w/v) Sodium Azide |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Alternative Name: | 5-hydroxymethylcytidine, anti-5-hydroxymethylcytidine, anti-5-hydroxymethylcytosine, anti-5hmC, anti-5-hmC, anti-5-hmeC, anti-5hmeC, 5hmC, 5hmeC, 5-hmeC |
| Scientific Background: | 5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells). Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0001 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0209 | 5-Hydroxymethyluridine Polyclonal Antibody | Inquiry |
| EAb-0226 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0254 | 5-Methylcytosine (5-mC) Monoclonal Antibody (RM231) | Inquiry |
| EAb-0255 | 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| 5-caC | 5-fC | 5-hmC | 5-Hydroxymethyluridine |
| 5-mC | 5-mCpA | ||
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