| Cat.No.: | EAb-0226 |
| Product Overview: | Rabbit polyclonal to detect 5-hydroxymethylcytosine (5-hmC) |
| Description: | Recent advances in the field of epigenetics have identified 5-hydroxymethylcytosine (5-hmC) as a key factor in the regulation of gene expression, with substantial implications in the study of tissue differentiation, neurological development, and carcinogenesis. Studies of this epigenetic marker are typically confounded by a lack of reliable methodology for differentiation from the highly prevalent 5-methylcytosine in a DNA sample. The 5-hydroxymethylcytosine pAb has been developed in order to robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA. Specificity of the antibody is enhanced such that crossreactivity with unmodified and methylated templates is suppressed to near-background levels. The antibody has been extensively tested and validated in ELISA and immunoprecipitation-based enrichment assays, and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting. Overall, the 5-hydroxymethylcytosine pAb has low cross reactivity with cytosine and 5-methylcytosine versus other available antibodies; can be used with a variety of genomic DNA sources; and has high sensitivity to low masses of 5-hydroxymethylcytosine DNA. |
| Antibody Type: | Polyclonal |
| Immunogen: | 5-Hydroxymethylcytosine |
| Host: | Rabbit |
| Isotype: | IgG1 |
| Antibody Target: | 5-hmC |
| Specificity: | Recognizes 5-hydroxymethylcytosine in single-stranded DNA from any mammalian, plant, insect, or microbial sources, as well as artificial templates used for standardization. |
| Purification: | Protein A Chromatography |
| Appearance: | Liquid |
| Formulation: | In PBS, pH 7.5, containing 0.05% sodium azide. |
| Applications: | ELISA, IP, Immunoblot |
| Recommended Dilutions/Conditions: |
ELISA (1:1,000-5,000); Immunoblotting (Dot blotting, 1:500-2,000); Immunoprecipitation (1-2 µg). Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Species independent |
| Shipping: | Blue Ice |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Alternative Name: | 5-hydroxymethylcytosine |
| Scientific Background: | DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0001 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0209 | 5-Hydroxymethyluridine Polyclonal Antibody | Inquiry |
| EAb-0226 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0254 | 5-Methylcytosine (5-mC) Monoclonal Antibody (RM231) | Inquiry |
| EAb-0255 | 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| 5-caC | 5-fC | 5-hmC | 5-Hydroxymethyluridine |
| 5-mC | 5-mCpA | ||
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