| Cat.No.: | EAb-0001 |
| Product Overview: | Polyclonal Antibody to detect 5-hmC in human samples. |
| Antibody Type: | Polyclonal |
| Immunogen: | Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH. |
| Host: | Rabbit |
| Isotype: | IgG |
| Antibody Target: | 5-hmC |
| Purification: | Whole antiserum |
| Appearance: | Liquid |
| Formulation: | In PBS with 0.05% (W/V) sodium azide. |
| Applications: | ELISA, hmEdIP, Dot blot. |
| Recommended Dilutions/Conditions: |
hMeDIP: 2.5 µg/IP, Dot Blot: 1:200, ELISA: 1:500. Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Positive Control: | ELISA: Peptide, hmEdIP, Dot blot: Peptides. |
| Species Reactivity: | Human, mouse, wide range |
| Shipping: | Gel Pack |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Alternative Name: | 5-hydroxymethylcytosine |
| Scientific Background: | 5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0.6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is abundant in brain tissue, especially in areas that are associated with higher cognitive functions. Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0001 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0209 | 5-Hydroxymethyluridine Polyclonal Antibody | Inquiry |
| EAb-0226 | 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody | Inquiry |
| EAb-0254 | 5-Methylcytosine (5-mC) Monoclonal Antibody (RM231) | Inquiry |
| EAb-0255 | 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| 5-caC | 5-fC | 5-hmC | 5-Hydroxymethyluridine |
| 5-mC | 5-mCpA | ||
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