| Cat.No.: | EL-0118 |
| Product Name: | Recombinant Mouse CDK1 Cell Lysate |
| Product Overview: | Mouse CDC2 Kinase / CDK1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer. |
| Species: | Mouse |
| Source: | Baculovirus-Insect Cells |
| Lysis Buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Recommended Usage: | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage: | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Gene ID NCBI: | 12534 |
| Official Symbol: | CDK1 |
| Official Full Name: | cyclin-dependent kinase 1 |
| Synonyms: | CDK1; cyclin-dependent kinase 1; p34 protein kinase; cell division protein kinase 1; cell division cycle 2 homolog A; cell cycle p34 CDC2 kinase protein; cell division cycle control protein 2a; cell division control protein 2 homolog; Cdc2; Cdc2a; p34< cdc2=""> |
| mRNA Refseq: | NM_007659 |
| Protein Refseq: | NP_031685 |
| Function: | ATP binding; Hsp70 protein binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups; |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0112 | Human CDKN1B Knockout Cell Line 10bp deletion | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0118 | Recombinant Mouse CDK1 Cell Lysate | Inquiry |
| EL-0119 | Recombinant Human CDK1 Cell Lysate | Inquiry |
| EL-0209 | Recombinant Human CD1D & B2M Cell Lysate | Inquiry |
| EL-0210 | Recombinant Human CD1D Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| CD1D | CDA | CDC34 | CDK1 |
| CDK8 | CDK9 | CDKA1 | CDKN1B |
| CDX1 | CDY2A | CDYL | |
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