| Cat.No.: | EL-0061 |
| Product Name: | Recombinant Human HES1 293 Cell Lysate |
| Description: | Antigen standard for hairy and enhancer of split 1, (Drosophila) (HES1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
| Species: | Human |
| Source: | HEK 293 Cells |
| Components: | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
| Storage: | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Gene ID NCBI: | 3280 |
| Official Symbol: | HES1 |
| Official Full Name: | hairy and enhancer of split 1 |
| Synonyms: | HES1; hairy and enhancer of split 1, (Drosophila); hairy homolog (Drosophila) , HRY; transcription factor HES-1; bHLHb39; FLJ20408; HES 1; Hes1; hairy homolog; hairy-like protein; class B basic helix-loop-helix protein 39; HHL; HRY; HES-1; |
| mRNA Refseq: | NM_005524 |
| Protein Refseq: | NP_005515 |
| MIM: | 139605 |
| UniProt ID: | Q14469 |
| Chromosome Location: | 3q28-q29 |
| Function: | DNA binding; N-box binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; histone deacetylase binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription factor binding; |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0061 | Recombinant Human HES1 293 Cell Lysate | Inquiry |
| EL-0112 | Recombinant Human HEY2 293 Cell Lysate | Inquiry |
| ◆ Cell Lines | ||
| CL-0078 | Human HELLS Knockout Cell Line 1bp insertion | Inquiry |
| ◆ Antibodies | ||
| EAb-0363 | HELLS Polyclonal Antibody | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-0534 | Recombinant Human HES1 | Inquiry |
| Related Gene / Proteins | |||
| HELLS | HELZ | HEMK1 | HERC3 |
| HERC6 | HES1 | HEY2 | |
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