| Cat.No.: | EL-0026 |
| Product Name: | Recombinant Human EED Cell Lysate |
| Product Overview: | Human EED / Embryonic Ectoderm Development derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer. |
| Species: | Human |
| Source: | Baculovirus-Insect Cells |
| Lysis Buffer: | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Recommended Usage: | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage: | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Gene ID NCBI: | 8726 |
| Official Symbol: | EED |
| Official Full Name: | embryonic ectoderm development |
| Synonyms: | EED; embryonic ectoderm development; polycomb protein EED; HEED; WAIT 1; WD protein associating with integrin cytoplasmic tails 1; WAIT1; |
| mRNA Refseq: | NM_003797 |
| Protein Refseq: | NP_003788 |
| MIM: | 605984 |
| UniProt ID: | O75530 |
| Chromosome Location: | 11q14.2-q22.3 |
| Function: | chromatin binding; histone methyltransferase activity; identical protein binding; protein binding; |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0026 | Recombinant Human EED Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0166 | MAK-683 | Inquiry |
| ◆ Research Kits | ||
| EKIT-0182 | EZH2-EED Binding Assay Kit | Inquiry |
| ◆ Antibodies | ||
| EAb-0196 | EEF1A Polyclonal Antibody | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-0248 | Recombinant Human EED, His & GST tagged | Inquiry |
| Related Gene / Proteins | |||
| EED | EEF1A1 | EEF1A2 | EEF1B2 |
| EEF1G | |||
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