| Cat.No.: | EKIT-0546 |
| Product Name: | PHF8 (KDM7B) Homogeneous Assay Kit |
| Size: | 384 reactions |
| Description: | The PHF8 Homogeneous Assay Kit is designed to measure the activity of PHF8 for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 on histone H3. The PHF8 Homogeneous Assay Kit comes in a convenient AlphaLISA format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified PHF8 for 384 enzyme reactions. The key to the PHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing PHF8 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. |
| Applications: | For studying enzyme kinetics and HTS applications. |
| Kit Components: | The PHF8 (KDM7B) Homogeneous Assay Kit comes in a convenient AlphaLISA format with primary antibody 17-4, FLAG-tagged PHF8, and assay buffers for 384 reactions. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
| ◆ Antibodies | ||
| EAb-0056 | PHF8 Polyclonal Antibody | Inquiry |
| EAb-0058 | PHC2 Polyclonal Antibody | Inquiry |
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| KDM1 | KDM1A | KDM1B | KDM2 |
| KDM2A | KDM2B | KDM3A | KDM3B |
| KDM4 | KDM4A | KDM4B | KDM4C |
| KDM4D | KDM4E | KDM5 | KDM5A |
| KDM5B | KDM5C | KDM5D | KDM6A More > |
| KDM6B | KDM6C | KDM7 | KDM7A |
| KDM7B | KDM8 | PHB | PHC1 |
| PHC2 | PHD | PHD1 | PHD2 |
| PHD3 | PHF1 | PHF10 | PHF13 |
| PHF17 | PHF2 | PHF20 | PHF20B |
| PHF21A | PHF21B | PHF6 | PHF8 |
| PHIP | PHPT1 | ||
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