| Cat.No.: | EKIT-0473 |
| Product Name: | JHDM1D Homogeneous Assay Kit |
| Size: | 96 well |
| Description: | The JHDM1D Homogeneous Assay Kit is designed to measure the activity of the JHDM1D for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JHDM1D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. |
| Applications: | For studying enzyme kinetics and HTS applications. |
| Kit Components: | The JHDM1D Homogeneous Assay Kit comes in a convenient AlphaLISA format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JHDM1D for 100 enzyme reactions. |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0470 | JHDM1D (KDM7A) Chemiluminescent Assay Kit | Inquiry |
| EKIT-0472 | JHDM1D (KDM7A) Homogeneous Assay Kit | Inquiry |
| EKIT-0473 | JHDM1D Homogeneous Assay Kit | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-1795 | JHDM1D (KDM7A), FLAG-tag | Inquiry |
| PE-1799 | FBXL11 (KDM2A, JHDM1A), FLAG-Avi-tags | Inquiry |
| Related Gene / Proteins | |||
| JHDM1A | JHDM1B | JHDM1D | JHDM2A |
| JHDM3A | |||
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