| Cat.No.: | EKIT-0453 |
| Assay Type: | Quantitative |
| Description: | Chromatin Accessibility Assay Kit is a complete set of optimized reagents designed for conducting a gene-specific analysis of chromatin accessibility including nucleosome/transcription factor positioning from various biological samples via real time PCR.The chromatin accessibility assay works on the principle that accessible chromatin is easily accessed by a nuclease mix to digest the DNA. When the DNA from the nuclease-treated sample and a no-nuclease control is analyzed for a particular gene by PCR, the more accessible the chromatin at that gene, the lower the signal of the nuclease-treated sample relative to the no-nuclease control. |
| Background: | The accessibility of regulatory elements in chromatin is critical for many aspects of gene regulation. Nucleosomes positioned over regulatory elements inhibit access of transcription factors to DNA. To elucidate the role of the interactions between chromatin and transcription factors, it is crucial to determine chromatin accessibility through mapping of the nucleosome positioning along the genome. In general, the more condensed the chromatin, the more difficult it is for transcription factors and other DNA binding proteins to access DNA and carry out their tasks. The more accessible the DNA, the more likely surrounding genes are actively transcribed. The presence (or the absence) of nucleosomes directly or indirectly affects a variety of other cellular and metabolic processes such as recombination, replication, centromere formation, and DNA repair. |
| Target Species: | Mammals |
| Warning: | For research use only, not for diagnostic use. |
| Product Types | ||
| ◆ Research Kits | ||
| EKIT-0001 | Acetyl Histone H3-K14 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0002 | Acetyl Histone H3-K14 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0003 | Acetyl Histone H3-K18 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0004 | Acetyl Histone H3-K18 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0005 | Acetyl Histone H3-K23 Quantification Kit (Colorimetric) | Inquiry |
USA
Quick Links
Easy access to products and services you need from our library via powerful searching tools
Privacy Policy | Cookie Policy
Copyright © 2026 CD BioSciences. All Rights Reserved.
