PRMT6 (HRMT1L6) Chemiluminescent Assay Kit

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Cat.No.:  EKIT-0281
Product Name:  PRMT6 (HRMT1L6) Chemiluminescent Assay Kit
Size:  96 reactions
Product Overview:  PRMT6 is a histone methyltransferase that exhibits methylation activity toward H3-R2 and H4-R3.
Description:  The PRMT6 Direct Activity Assay kit is designed to measure PRMT6 activity for screening and profiling applications. The key to the PRMT6 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Kit Components:  PRMT6 human recombinant enzyme: 20 µg; -80°C 20 µM S-adenosylmethionine*: 250 µl; -80°C Primary antibody 4: 100 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C4x HMT assay buffer 6: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C 96-well plate precoated with histone substrate: 1 plate; +4°C* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.
Warning:  For research use only, not for diagnostic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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