| Description: |
CUT&Tag (Cleavage Under Targets and Tagmentation) is a contemporary and sensitive technique for profiling chromatin-associated proteins and histone modifications, offering superior signal-to-noise ratio and lower input requirements compared to traditional ChIP-seq. To maximize its potential and accessibility, we have developed the Chromatin DNA-Protein Interaction Analyzer. This fully automated system transforms the multi-step, manual CUT&Tag protocol into a streamlined, push-button workflow. Simply load your prepared cell or nuclei suspension, and the instrument automatically executes the entire process—from cell capture and antibody incubation to tagmentation and library amplification—culminating in the generation of high-quality, sequencing-ready CUT&Tag libraries. It ensures exceptional reproducibility, minimizes hands-on time, and enables researchers to consistently obtain robust epigenetic data from precious biological samples.
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| Workflow: |
• Cell Capture
• Antibody Incubation
• Transposase Incubation
• DNA Fragment Recovery
• PCR Reaction Solution Preparation
• Library Purification
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| Sample Types: |
Animal cells, animal cell nuclei, plant cell nuclei, cell nuclei cross-linked with 0.01%-0.3% formaldehyde
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| Sample Throughput: |
1-16 samples can be processed simultaneously
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| Antibodies: |
Supports 1-4 primary antibodies and 1-2 secondary antibodies
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| Cell Sample Quantity: |
Each sample contains 500-50,000 cells (nuclei)
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| Library Construction Time: |
Library construction of 1-16 samples can be completed within 7.5 hours
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| Reagents: |
Pre-fabricated reagent cartridges, requiring almost no manual operation
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| Temperature Control Module: |
The reagent storage area supports cooling to 4°C, and the reaction area supports heating from room temperature to 60°C. The heating module adopts a bottom-fully enclosed and side-partially enclosed heating method, which ensures rapid heating and strong temperature stability.
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| Magnetic Module: |
Permanent magnet column, 4000Gs~6000Gs; magnetic bead recovery rate, >98%
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| User Interface: |
10.1-inch full-color LCD touchscreen
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| Illumination: |
Built-in LED lights, freely switchable via the screen
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| Contamination Control: |
Built-in UV disinfection module
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| Note: |
The instrument has functions such as self-test upon startup, silent operation, power failure protection, and over-temperature protection, which can minimize sample loss during instrument use.
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