| Cat.No.: | PE-3438 |
| Product Name: | Recombinant METTL16 protein |
| Product Overview: | Recombinant human METTL16 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_076991.3) with an N-terminal FLAG tag. The molecular weight of the protein is 64.9 kDa. |
| Background: | METTL16 (Methyltransferase-like protein 16), also known as U6 small nuclear RNA (adenine-(43)-N(6))- methyltransferase or METT10D (Methyltransferase 10 Domain-Containing Protein), is a RNA N6-methyltransferase that methylates adenosine residues of a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, METTL16 is only able to methylate a limited number of RNAs: requires both a 5UACAGAGAA-3 nonamer sequence and a specific RNA structure. It also plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. In presence of S-adenosyl-L-methionine, METTL16 binds the 3-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing. While in S-adenosyl-L-methionine-limiting conditions, it binds the 3-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A. |
| Applications: | Suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. |
| Tag: | FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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| ◆ Antibodies | ||
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| ◆ Cell Lines | ||
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