Chromatin DNA-Protein Interaction Analyzer

CUT&Tag technology has redefined chromatin profiling by delivering high-resolution protein-DNA interaction maps with exceptional signal clarity and minimal sample input. Now, CD BioSciences advances this revolution with the chromatin DNA-protein interaction analyzer—an integrated, automated platform designed to transform this powerful method into a routine, robust, and reproducible workflow. From antibody-guided targeting to sequencing-ready libraries, we bring standardized, publication-quality epigenomics within reach of every researcher.

Introduction to CUT&Tag

Cleavage under targets and tagmentation (CUT&Tag) is a high-efficiency, high-resolution epigenomic technique for profiling protein-DNA interactions. Its principle is based on targeted chromatin cleavage and tagmentation: a specific antibody first recognizes the target protein (such as a histone modification or transcription factor), which then guides the Tn5 transposase to cleave the nearby DNA and insert sequencing adapters at the binding site. The fragmented DNA is subsequently amplified and sequenced, enabling precise mapping of genomic regions bound or modified by the protein. This technique offers the advantages of low sample input (down to single cells), high signal-to-noise ratio, and a short experimental timeline. It is widely used for mapping histone modifications (e.g., H3K4me3, H3K27ac), deciphering transcription factor regulatory networks (e.g., pluripotency factors OCT4, SOX2), revealing disease-associated chromatin state changes (e.g., aberrant enhancer activation in cancer), and investigating epigenetic regulatory mechanisms of gene expression in conjunction with multi-omics approaches.

Fig.1 The basic principle of CUT & Tag.

Our Instruments

To liberate CUT&Tag technology from complex manual protocols and enable efficient, standardized, and reproducible chromatin interaction analysis, CD BioSciences proudly introduces the chromatin DNA-protein interaction analyzer. This integrated system automates the entire workflow—from sample processing to targeted cleavage and tagmentation, through final library preparation. Starting with cell samples, the instrument seamlessly executes all experimental steps to directly generate high-quality, sequencing-ready CUT&Tag libraries, dramatically simplifying the process and ensuring exceptional data reliability and consistency.

Overview of Instrument Functions

After loading cell or nuclear samples, our chromatin DNA-protein interaction analyzer automates the entire process, producing CUT&Tag libraries ready for direct sequencing.

1. Supported Sample Types: It supports various sample types, including animal cells, plant and animal cell nuclei, and nuclei cross-linked with 0.01%-0.3% formaldehyde.
2. Complementary Products: The accompanying CUT&Tag kit includes cell capture magnetic beads, various buffers, Tn5 transposase, library purification magnetic beads, PCR Mix, Index primers, and other necessary reagents.

Fig.2 Workflow of the chromatin DNA-protein interaction analyzer.

Instrument Parameters

* Note: The instrument has functions such as self-test upon startup, silent operation, power failure protection, and over-temperature protection, which can minimize sample loss during instrument use.

Compatible Reagent Kits

The kit contains all the necessary reagents, including cell capture magnetic beads, various buffers, Tn5 transposase, library purification magnetic beads, PCR Mix, and Index primers.

* Note: The primary and secondary antibodies need to be provided by the user.

Example of Instrument Measurement Results

1. Chromatin Mapping

Using the chromatin DNA-protein interaction analyzer, high-quality chromatin maps of various transcription factors and histone modification targets can be obtained. This eliminates the need for complex manual operations, saving researchers significant time and effectively improving work efficiency.

Fig.3 Chromatin map obtained using the chromatin DNA-protein interaction analyzer.

1. CUT&Tag Experiment of HeLa Cells

Product Advantages

1. Full Automation & Standardization: Automates the entire CUT&Tag workflow—from cell handling to sequencing-ready library preparation—eliminating manual variability and ensuring exceptional reproducibility.
2. High Consistency & Superior Data Quality: Delivers highly consistent CUT&Tag profiles with excellent signal-to-noise ratio, reliably outperforming manual methods in data uniformity and accuracy.
3. Rapid & Efficient Processing: Dramatically reduces experimental time, completing library preparation in a fraction of the time required by conventional manual protocols.
4. Low-Input Flexibility: Supports a wide range of input materials, from single cells to larger populations, making it ideal for both scarce samples and high-throughput studies.

As a leading provider in epigenetics research solutions, CD BioSciences offers a comprehensive portfolio, including proteins & enzymes, antibodies, synthetic peptides, cell lines, nucleosomes, research kits, and advanced instruments, to empower every stage of your scientific discovery. If you are interested in our products, please do not hesitate to contact us.

Reference

1. Orsburn B C. Simultaneous single-cell proteomics and epigenetic analysis of histone deacetylase inhibition in human cells[J]. Communications Biology, 2025.

• 

  CTLP-LJX-0001

  Chromatin DNA-Protein Interaction Analyzer